Enhanced Recognition and Neutralization of HIV-1 by Antibody-Derived CCR5-Mimetic Peptide Variants

被引：21

作者：

Chiang, Jessica J. ^{[1
]}

Gardner, Matthew R. ^{[1
]}

Dorfman, Tatyana ^{[1
]}

Choe, Hyeryun ^{[2
]}

Farzan, Michael ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, New England Primate Res Ctr, Dept Microbiol & Immunobiol, Southborough, MA 01772 USA

[2] Harvard Univ, Childrens Hosp, Sch Med, Dept Pediat, Boston, MA 02115 USA

来源：

JOURNAL OF VIROLOGY | 2012年 / 86卷 / 22期

关键词：

GP120 ENVELOPE GLYCOPROTEIN; N-TERMINUS; TYROSINE SULFATION; AMINO-TERMINUS; CCR5; RECEPTOR; ENTRY; CD4; INFECTION; BINDING;

D O I：

10.1128/JVI.00967-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A tyrosine-sulfated CCR5-mimetic peptide, CCR5mim1, inhibits HIV-1 infection more efficiently than sulfopeptides based on the CCR5 amino terminus. Here we characterized sulfopeptide chimeras of CCR5mim1 and the heavy-chain CDR3 of the antibody PG16. Two chimeras bound a range of envelope glycoproteins and neutralized HIV-1 more efficiently than CCR5mim1. An immunoadhesin form of one of these, CCR5mim2-Ig, synergized with CD4-Ig to neutralize HIV-1. These sulfopeptides are among the broadest and most potent CCR5-mimetic peptides described to date.

引用

页码：12417 / 12421

页数：5

共 24 条

[1] The beta-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates [J].

Choe, H ;

Farzan, M ;

Sun, Y ;

Sullivan, N ;

Rollins, B ;

Ponath, PD ;

Wu, LJ ;

Mackay, CR ;

LaRosa, G ;

Newman, W ;

Gerard, N ;

Gerard, C ;

Sodroski, J .

CELL, 1996, 85 (07) :1135-1148

[2] Tyrosine sulfation of human antibodies contributes to recognition of the CCR5 binding region of HIV-1 gp120 [J].

Choe, H ;

Li, WH ;

Wright, PL ;

Vasilieva, N ;

Venturi, M ;

Huang, CC ;

Grundner, C ;

Dorfman, T ;

Zwick, MB ;

Wang, LP ;

Rosenberg, ES ;

Kwong, PD ;

Burton, DR ;

Robinson, JE ;

Sodroski, JG ;

Farzan, M .

CELL, 2003, 114 (02) :161-170

[3] Specific interaction of CCR5 amino-terminal domain peptides containing sulfotyrosines with HIV-1 envelope glycoprotein gp120 [J].

Cormier, EG ;

Persuh, M ;

Thompson, DAD ;

Lin, SW ;

Sakmar, TP ;

Olson, WC ;

Dragic, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (11) :5762-5767

[4] The crown and stem of the V3 loop play distinct roles in human immunodeficiency virus type 1 envelope glycoprotein interactions with the CCR5 coreceptor [J].

Cormier, EG ;

Dragic, T .

JOURNAL OF VIROLOGY, 2002, 76 (17) :8953-8957

[5] THE CD4 (T4) ANTIGEN IS AN ESSENTIAL COMPONENT OF THE RECEPTOR FOR THE AIDS RETROVIRUS [J].

DALGLEISH, AG ;

BEVERLEY, PCL ;

CLAPHAM, PR ;

CRAWFORD, DH ;

GREAVES, MF ;

WEISS, RA .

NATURE, 1984, 312 (5996) :763-767

[6] A tyrosine-sulfated peptide derived from the heavy-chain CDR3 region of an HIV-1-neutralizing antibody binds gp120 and inhibits HIV-1 infection [J].

Dorfman, Tatyana ;

Moore, Michael J. ;

Guth, Alexander C. ;

Choe, Hyeryun ;

Farzan, Michael .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (39) :28529-28535

[7] A tyrosine-sulfated peptide based on the N terminus of CCR5 interacts with a CD4-enhanced epitope of the HIV-1 gp120 envelope glycoprotein and inhibits HIV-1 entry [J].

Farzan, M ;

Vasilieva, N ;

Schnitzler, CE ;

Chung, S ;

Robinson, J ;

Gerard, NP ;

Gerard, C ;

Choe, H ;

Sodroski, J .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (43) :33516-33521

[8] Tyrosine sulfation of the amino terminus of CCR5 facilitates HIV-1 entry [J].

Farzan, M ;

Mirzabekov, T ;

Kolchinsky, P ;

Wyatt, R ;

Cayabyab, M ;

Gerard, NP ;

Gerard, C ;

Sodroski, J ;

Choe, H .

CELL, 1999, 96 (05) :667-676

[9] The role of post-translational modifications of the CXCR4 amino terminus in stromal-derived factor 1α association and HIV-1 entry [J].

Farzan, M ;

Babcock, GJ ;

Vasilieva, N ;

Wright, PL ;

Kiprilov, E ;

Mirzabekov, T ;

Choe, H .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (33) :29484-29489

[10] A tyrosine-rich region in the N terminus of CCR5 is important for human immunodeficiency virus type 1 entry and mediates an association between gp120 and CCR5 [J].

Farzan, M ;

Choe, H ;

Vaca, L ;

Martin, K ;

Sun, Y ;

Desjardins, E ;

Ruffing, N ;

Wu, LJ ;

Wyatt, R ;

Gerard, N ;

Gerard, C ;

Sodroski, J .

JOURNAL OF VIROLOGY, 1998, 72 (02) :1160-1164

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