Analysis of the methylation status of genes up-regulated by the demethylating agent, 5-aza-2′-deoxycytidine, in esophageal squamous cell carcinoma

被引：18

作者：

Arai, Makoto ^{[1
,2
]}

Imazeki, Fumio ^{[1
]}

Sakai, Yuzo ^{[2
]}

Mikata, Rintaro ^{[1
]}

Tada, Motohisa ^{[1
]}

Seki, Naohiko ^{[3
]}

Shimada, Hideaki ^{[4
]}

Ochiai, Takenori ^{[4
]}

Yokosuka, Osamu ^{[1
]}

机构：

[1] Chiba Univ, Grad Sch Med, Dept Med & Clin Oncol, Chiba 2608670, Japan

[2] Chiba Univ, Grad Sch Med, 21st Century Ctr Excellence COE Program, Chiba 2608670, Japan

[3] Chiba Univ, Grad Sch Med, Dept Funct Genom, Chiba 2608670, Japan

[4] Chiba Univ, Grad Sch Med, Acad Dept Surg, Chiba 2608670, Japan

来源：

ONCOLOGY REPORTS | 2008年 / 20卷 / 02期

关键词：

methylation; esophageal squamous cell carcinoma; microarray; normal mucosa of esophagus specific 1;

D O I：

10.3892/or_00000022

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

To determine the clinical significance of gene promoter methylation in esophageal squamous cell carcinoma (ESCC), we examined the promoter methylation status of genes showing elevated expression, as determined by DNA microarray-based transcriptomic analysis, in three ESCC cell lines (TE-1, TE-2, TE-10) after 5-aza-2'-deoxycytidine (DAC) treatment. We observed a high degree of DNA methylation within the promoter regions of three genes, namely cathepsin L2 (CTSL2), normal mucosa of esophagus specific 1 (NMES1), and fatty acid binding protein 5 (FABP5). Overexpression of NMES1 in ESCC cell lines increased cell motility. Downregulation of NMES1 might play an important role in the cell motility of ESCC or be a potent marker of malignancy.

引用

页码：405 / 412

页数：8

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