Elevation of plasma thioredoxin levels in HIV-infected individuals

被引:174
作者
Nakamura, H
DeRosa, S
Roederer, M
Anderson, MT
Dubs, JG
Yodoi, J
Holmgren, A
Herzenberg, LA
Herzenberg, LA
机构
[1] STANFORD UNIV,SCH MED,DEPT GENET,STANFORD,CA 94305
[2] KYOTO UNIV,DEPT BIOL RESPONSES,INST VIRUS RES,KYOTO 60601,JAPAN
[3] KAROLINSKA INST,MED NOBEL INST BIOCH,DEPT MED BIOCHEM & BIOPHYS,S-17177 STOCKHOLM,SWEDEN
关键词
adult T cell leukemia-derived factor; CD4; CD38; ELISA; glutathione; human; naive T cell; oxidative stress; redox; thioredoxin;
D O I
10.1093/intimm/8.4.603
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Thioredoxin (Trx), a ubiquitous protein intimately involved in redox and protein disulfide reductions, has been shown to be released from cells and to have cytokine-like activities. In addition, Trx has been implicated in the redox regulation of immunological responses and shown to be deficient in tissues from AIDS patients, In studies presented here, plasma Trx levels were measured by ELISA in plasma samples from HIV-infected individuals (n = 136) and HIV-negative controls (n = 47), To account for the release of Trx into plasma due to hemolysis, the Trx measurements were corrected according to the level of hemoglobin in the plasma sample. Data presented show that, in contrast to tissue Trx levels, corrected plasma Trx levels are significantly higher in HIV-infected individuals than in controls (P < 0.0001). furthermore, similar to 25% of the HIV-infected individuals studied have plasma Trx levels greater than the highest level found in controls (37 ng/ml), Detailed multiparameter FAGS analysis of peripheral blood mononuclear cells (PBMC) from the infected individuals demonstrates that those with higher plasma Trx levels (37 ng/ml or greater) tend to have lower overall CD4 counts. In addition, increases in plasma Trx levels correlate with decreases in monochlorobimane staining (indicative of lower intracellular glutathione levels in PBMC) and with changes in surface antigen expression (CD62L, CD38 and CD20) that occur in the later stages of HIV infection. These correlations suggest that elevation of plasma Trx levels may be an important component of advanced HIV disease, perhaps related to the oxidative stress that often occurs at this stage.
引用
收藏
页码:603 / 611
页数:9
相关论文
共 51 条
  • [1] AMEISEN JC, 1991, IMMUNOL TODAY, V12, P102
  • [2] ANDERSON MT, 1996, IN PRESS HDB EXPT IM
  • [3] ENZYMATIC REDUCTION OF DISULFIDE BONDS IN FIBRIN-OGEN BY THIOREDOXIN SYSTEM .1. IDENTIFICATION OF REDUCED BONDS AND STUDIES ON REOXIDATION PROCESS
    BLOMBACK, B
    BLOMBACK, M
    FINKBEINER, W
    HOLMGREN, A
    KOWALSKA.B
    OLOVSON, G
    [J]. THROMBOSIS RESEARCH, 1974, 4 (01) : 55 - 75
  • [4] CLINICAL UTILITY OF LIVER-BIOPSY IN PATIENTS WITH SERUM ANTIBODIES TO THE HUMAN-IMMUNODEFICIENCY-VIRUS
    CAPPELL, MS
    SCHWARTZ, MS
    BIEMPICA, L
    [J]. AMERICAN JOURNAL OF MEDICINE, 1990, 88 (02) : 123 - 130
  • [5] CAPPELL MS, 1991, AM J GASTROENTEROL, V86, P1
  • [6] CLARKE FM, 1991, J REPROD FERTIL, V93, P525, DOI 10.1530/jrf.0.0930525
  • [7] DEISS A, 1993, WINTROBES CLIN HEMAT, P195
  • [8] GLUTATHIONE DEPLETION IN HIV-INFECTED PATIENTS - ROLE OF CYSTEINE DEFICIENCY AND EFFECT OF ORAL N-ACETYLCYSTEINE
    DEQUAY, B
    MALINVERNI, R
    LAUTERBURG, BH
    [J]. AIDS, 1992, 6 (08) : 815 - 819
  • [9] LOW CONCENTRATIONS OF ACID-SOLUBLE THIOL (CYSTEINE) IN THE BLOOD-PLASMA OF HIV-1-INFECTED PATIENTS
    ECK, HP
    GMUNDER, H
    HARTMANN, M
    PETZOLDT, D
    DANIEL, V
    DROGE, W
    [J]. BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1989, 370 (02): : 101 - 108
  • [10] THIOREDOXIN FROM ACTIVATED MACROPHAGES AS A TROPHIC FACTOR FOR CENTRAL CHOLINERGIC NEURONS INVITRO
    ENDOH, M
    KUNISHITA, T
    TABIRA, T
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 192 (02) : 760 - 765