Oligo-DNA Functionalized Polyorganosiloxane Nanoparticles

被引：5

作者：

Diehl, Christina ^{[1
]}

Fluegel, Sabine ^{[1
]}

Fischer, Karl ^{[1
]}

Maskos, Michael ^{[1
]}

机构：

[1] Johannes Gutenberg Univ Mainz, Inst Phys Chem, D-55128 Mainz, Germany

来源：

SURFACE AND INTERFACIAL FORCES - FROM FUNDAMENTALS TO APPLICATIONS | 2008年 / 134卷

关键词：

Cellular uptake; Nanoparticles; Oligo-DNA; PEO; Polyorganosiloxane;

D O I：

10.1007/2882_2008_086

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Different oligo-DNA surface functionalized polyorganosiloxane nanoparticles have been synthesized and characterized. The core-shell polyorganosiloxane nanoparticles possess a hydrodynamic radius of 14.1 (sample code CS I) and 12.1 nm (sample code CS2), respectively. Coupling of different short chain poly(ethylene oxide)s resulted in water soluble nanospheres. For CS2-PEO110, a number of approximately 500 PEO per siloxane nanosphere has been determined. Two different (partially fluorescently labelled) oligo-DNA sequences (12 and 20 bases, respectively) have been coupled to the PEO grafted on the nanoparticles with high coupling efficiency. MDCKII cells were incubated using a solution of these bio-hybrid nanoparticles and the successful intracellular uptake was visualized by fluorescence microscopy.

引用

页码：128 / 133

页数：6

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