Qa-1b and CD94-NKG2a interaction regulate cytolytic activity of herpes simplex virus-specific memory CD8+ T cells in the latently infected trigeminal ganglia

被引:59
作者
Suvas, S [1 ]
Azkur, AK [1 ]
Rouse, BT [1 ]
机构
[1] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA
关键词
D O I
10.4049/jimmunol.176.3.1703
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
After ocular infection, HSV-specific CD8(+) T cells migrate to and are specifically retained in the ophthalmic branch of the trigeminal ganglia (TG) even at the time when replicating virus is no longer evident. Virus-specific CD8(+) T cells maintain an activation phenotype and secrete IFN-gamma in the latent TG. In this report we demonstrated that activated virus-specific memory CD8(+) T cells, although potentially cytolytic, also expressed the CD94-NK cell receptor subfamily G2a inhibitory molecule and were unable to exert cytotoxicity when engaged by Qa-1(h) expressing targets. Interestingly, many neurons in the latent TG expressed Qa-1(b), and blocking of Qa-1(b)/CD94-NKG2a interaction in an ex vivo TG culture resulted in neuronal cell lysis. The expression of the inhibitory CD94-NKG2a molecule could be induced by TGF-beta 1, which was shown to present as a bioactive molecule in the latent TG. Additionally, CD4(+) forkhead/winged helix transcription factor 3(+) T cells were also determined in the latent TG. Our results demonstrate the operation of a regulatory system in vivo that serves to protect irreplaceable neurons from destruction by the immune system.
引用
收藏
页码:1703 / 1711
页数:9
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