Oxidative folding of conotoxins in immobilized systems

被引：22

作者：

Green, BR ^{[1
]}

Bulaj, G ^{[1
]}

机构：

[1] Cognetix Inc, Salt Lake City, UT 84108 USA

来源：

PROTEIN AND PEPTIDE LETTERS | 2006年 / 13卷 / 01期

关键词：

conotoxins; oxidative folding; immobilized; CLEAR-OX (TM);

D O I：

10.2174/092986606774502162

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We tested two alternative oxidation strategies to produce conotoxins alpha-GI and mu-PIIIA. The peptides were either reversibly immobilized on a solid support and then oxidized, or the immobilized disulfide reagent (CLEAR-OX (TM)) was used to oxidize the peptides. Both strategies appeared more efficient at higher peptide concentrations, consistent with pseudo-dilution effects.

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页码：67 / 70

页数：4

相关论文

共 28 条

[1] Oxidative refolding chromatography:: folding of the scorpion toxin Cn5 [J].

Altamirano, MM ;

García, C ;

Possani, LD ;

Fersht, AR .

NATURE BIOTECHNOLOGY, 1999, 17 (02) :187-191

[2] Refolding chromatography with immobilized mini-chaperones [J].

Altamirano, MM ;

Golbik, R ;

Zahn, R ;

Buckle, AM ;

Fersht, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (08) :3576-3578

[3] Ligand-independent assembly of recombinant human CD1 by using oxidative refolding chromatography [J].

Altamirano, MM ;

Woolfson, A ;

Donda, A ;

Shamshiev, A ;

Briseño-Roa, L ;

Foster, NW ;

Veprintsev, DB ;

De Libero, G ;

Fersht, AR ;

Milstein, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (06) :3288-3293

[4] Novel solid-phase reagents for facile formation of intramolecular disulfide bridges in peptides under mild conditions [J].

Annis, I ;

Chen, L ;

Barany, G .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (29) :7226-7238

[5]

Annis I, 1997, METHOD ENZYMOL, V289, P198

[6] Propeptide does not act as an intramolecular chaperone but facilitates protein disulfide isomerase-assisted folding of a conotoxin precursor [J].

Buczek, O ;

Olivera, BM ;

Bulaj, G .

BIOCHEMISTRY, 2004, 43 (04) :1093-1101

[7] Efficient oxidative folding of conotoxins and the radiation of venomous cone snails [J].

Bulaj, G ;

Buczek, O ;

Goodsell, I ;

Jimenez, EC ;

Kranski, J ;

Nielsen, JS ;

Garrett, JE ;

Olivera, BM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 :14562-14568

[8] Facile preparation of disulfide-bridged peptides using the polymer-supported oxidant CLEAR-OX™ [J].

Darlak, K ;

Long, DW ;

Czerwinski, A ;

Darlak, M ;

Valenzuela, F ;

Spatola, AF ;

Barany, G .

JOURNAL OF PEPTIDE RESEARCH, 2004, 63 (03) :303-312

[9] Detergent-assisted oxidative folding of δ-conotoxins [J].

DeLa Cruz, R ;

Whitby, FG ;

Buczek, O ;

Bulaj, G .

JOURNAL OF PEPTIDE RESEARCH, 2003, 61 (04) :202-212

[10] Structure determination of the three disulfide bond isomers of α-conotoxin GI:: A model for the role of disulfide bonds in structural stability [J].

Gehrmann, J ;

Alewood, PF ;

Craik, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1998, 278 (02) :401-415