Reliable recovery of inbred mouse lines using cryopreserved spermatozoa

被引:34
作者
Marschall, S [1 ]
Huffstadt, U [1 ]
Balling, R [1 ]
de Angelis, MH [1 ]
机构
[1] GSF, Res Ctr Environm & Hlth, Inst Mammalian Genet, D-85764 Neuherberg, Germany
关键词
D O I
10.1007/s003359901090
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Since the mouse has become the most detailed model system to investigate the genetics and pathogenesis of human diseases, large numbers of new mouse strains have and continue to be produced. In nearly all animal facilities, the maintenance of breeding colonies is limited and mouse strains have to be archived in an efficient way. This study was undertaken to test the reliability of recovering mouse lines by use of cryopreserved spermatozoa from individual male mice. In contrast to many studies, spermatozoa and oocytes were derived from the same genetic background. 30 C3HeB/FeJ males belonging to three different categories (wild-type, F1-generation of ENU-treated males, and defined mutants) were recovered by producing at least 20 offspring from each donor. Independent of the experimental group, every single male was successfully recovered. Archiving mouse strains by cryopreservation of spermatozoa may, therefore, offer a reliable way to preserve genetically valuable mouse strains and provides an efficient management strategy for animal facilities.
引用
收藏
页码:773 / 776
页数:4
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