Detection of a variant metallo-β-lactamase, IMP-10, from two unrelated strains of Pseudomonas aeruginosa and an Alcaligenes xylosoxidans strain

被引：62

作者：

Iyobe, S

Kusadokoro, H

Takahashi, A

Yomoda, S

Okubo, T

Nakamura, A

O'Hara, K

机构：

[1] Gunma Univ, Sch Med, Lab Drug Resistance Bacteria, Maebashi, Gumma 3718511, Japan

[2] Gunma Univ, Sch Med, Clin Lab Ctr, Maebashi, Gumma 3718511, Japan

[3] Chiba Univ, Fac Pharmaceut Sci, Div Microbial Chem, Chiba, Japan

[4] Sci Univ Tokyo, Fac Pharmaceut Sci, Dept Microbial Chem, Tokyo, Japan

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2002年 / 46卷 / 06期

关键词：

D O I：

10.1128/AAC.46.6.2014-2016.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The gene bla(IMP-10) of a variant metallo-beta-lactamase, IMP-10, had a single base replacement of G by T at nucleotide 145, which led to an amino acid alteration of Val49 to Phe compared to the IMP-1 enzyme, indicating that IMP-10 was a point mutation derivative of IMP-1. Highly purified enzymes revealed that IMP-10 was different from IMP-1 in its extremely low hydrolyzing activities for penicillins, such as benzylpenicillin, ampicillin, and piperacillin.

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页码：2014 / 2016

页数：3

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