Transmembrane pores formed by synthetic p-octiphenyl β-barrels with internal carboxylate clusters:: Regulation of ion transport by pH and Mg2+ complexed 8-aminonaphthalene-1,3,6-trisulfonate

被引:32
作者
Das, G [1 ]
Matile, S [1 ]
机构
[1] Univ Geneva, Dept Organ Chem, CH-1211 Geneva, Switzerland
关键词
D O I
10.1073/pnas.022547899
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Design, synthesis, and study of a synthetic barrel-stave supramolecule with p-octiphenyl "staves," beta-sheet "hoops," and hydrophobic exterior as well as internal carboxylate clusters are reported. Ion transport experiments indicate the formation of transmembrane pores at 5 < pH < 7 with nanomolar activity. Blockage of dye efflux from spherical bilayers by external Mg(OAC)(2) and internal 8-aminonaphthalene-1,3,6-trisulfonate is suggestive for weakly cooperative (n = 1.16) formation of aspartate-Mg2+-8-aminonaphthalene-1,3,6-trisulfonate complexes within the barrel-stave supramolecule (K-D = 2.9 mM). Corroborative evidence from structural studies by circular dichroism spectroscopy is provided and discussed with emphasis of the importance of internal charge repulsion for pore formation and future applications toward binding and catalysis within supramolecular synthetic pores.
引用
收藏
页码:5183 / 5188
页数:6
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