Isolation and characterization of microsatellite loci from red sea bream Pagrus major and detection in closely related species

被引：65

作者：

Takagi, M ^{[1
]}

Taniguchi, N ^{[1
]}

Cook, D ^{[1
]}

Doyle, RW ^{[1
]}

机构：

[1] DALHOUSIE UNIV, DEPT BIOL, MARINE GENE PROBE LAB, HALIFAX, NS B3H 4J1, CANADA

来源：

FISHERIES SCIENCE | 1997年 / 63卷 / 02期

关键词：

microsatellite; Red sea bream; Pagrus major; Sparidae; genetic variability; population genetics;

D O I：

10.2331/fishsci.63.199

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Five (GT./CA)(n) microsatellite loci were isolated from a size-selected genomic library of red sea bream (Paqrus major). Primers for PCR amplification were constructed for the microsatellite loci and the loci characterized by screening polymorphisms in three wild populations of the red sea bream. All loci displayed a high degree of length polymorphism, as observed in the total number of alleles per locus (16-32), and a high degree of heterozygosity ranging from 0.675-0.907. Distinct differences were observed among three wild populations of red sea bream collected from coastal waters of Japan, both in the average number of alleles per locus and the frequency distributions of the alleles. The primers developed for red sea bream were also tested for their ability to amplify homologous sequences from 5 closely related species of Sparidae. These microsatellite loci show great potential as indicators for genetic variability and divergence among subpopulations of Pagrus major, and to a lesser degree with some of the related species tested.

引用

页码：199 / 204

页数：6

共 23 条

[1]

ALLENDORF F, 1976, HEREDITAS, V83, P73

[2]

AVICE JC, 1974, EVOLUTION, V28, P42

[3]

BROCKER AL, 1994, CAN J FISH AQUAT SCI, V51, P1959

[4]

DOYLE RW, 1995, P AQ S TECHN INV OPP, P602

[5] CHARACTERIZATION OF (GT)N AND (CT)N MICROSATELLITES IN 2 INSECT SPECIES - APIS-MELLIFERA AND BOMBUS-TERRESTRIS [J].

ESTOUP, A ;

SOLIGNAC, M ;

HARRY, M ;

CORNUET, JM .

NUCLEIC ACIDS RESEARCH, 1993, 21 (06) :1427-1431

[6] DNA FINGERPRINTING OF TILAPIA, OREOCHROMIS-NILOTICUS, AND ITS APPLICATION TO AQUACULTURE GENETICS [J].

HARRIS, AS ;

BIEGER, S ;

DOYLE, RW ;

WRIGHT, JM .

AQUACULTURE, 1991, 92 (2-3) :157-163

[7] DNA fingerprint based analysis of paternal and maternal effects on offspring growth and survival in communally reared rainbow trout [J].

Herbinger, CM ;

Doyle, RW ;

Pitman, ER ;

Paquet, D ;

Mesa, KA ;

Morris, DB ;

Wright, JM ;

Cook, D .

AQUACULTURE, 1995, 137 (1-4) :245-256

[8] INDIVIDUAL-SPECIFIC FINGERPRINTS OF HUMAN DNA [J].

JEFFREYS, AJ ;

WILSON, V ;

THEIN, SL .

NATURE, 1985, 316 (6023) :76-79

[9] HYPERVARIABLE MINISATELLITE REGIONS IN HUMAN DNA [J].

JEFFREYS, AJ ;

WILSON, V ;

THEIN, SL .

NATURE, 1985, 314 (6006) :67-73

[10]

LITT M, 1989, AM J HUM GENET, V44, P397

← 1 2 3 →