Functional DNAzymes organized into two-dimensional arrays

被引：36

作者：

Garibotti, Alejandra V.

Knudsen, Scott M.

Ellington, Andrew D.

Seeman, Nadrian C. ^{[1
]}

机构：

[1] NYU, Dept Chem, New York, NY 10003 USA

[2] Univ Texas, Dept Chem & Biochem, Austin, TX 78712 USA

来源：

NANO LETTERS | 2006年 / 6卷 / 07期

关键词：

IN-VITRO SELECTION; CLEAVING DNA ENZYME; CRYSTALS; MOLECULES;

D O I：

10.1021/nl0609955

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

DNAzymes are catalytically active DNA molecules, which have previously been described in solution. Here, we organize these molecules into a series of two-dimensional (2D) arrays using a periodic arrangement of DNA structures based on the DNA double crossover motif. We demonstrate by means of atomic force microscopy that the DNAzymes are organized according to the design and that they retain their activity when attached in linear strings within the context of the 2D array.

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收藏

页码：1505 / 1507

页数：3

相关论文

共 24 条

[1] DNA ribonucleases that are active against intracellular hepatitis B viral RNA targets [J].

Asahina, Y ;

Ito, Y ;

Wu, CH ;

Wu, GY .

HEPATOLOGY, 1998, 28 (02) :547-554

[2]

Breaker R R, 1994, Chem Biol, V1, P223, DOI 10.1016/1074-5521(94)90014-0

[3] A DNA ENZYME WITH MG2+-DEPENDENT RNA PHOSPHOESTERASE ACTIVITY [J].

BREAKER, RR ;

JOYCE, GF .

CHEMISTRY & BIOLOGY, 1995, 2 (10) :655-660

[4] Cleaving DNA with DNA [J].

Carmi, N ;

Balkhi, SR ;

Breaker, RR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (05) :2233-2237

[5] In vitro selection of self-cleaving DNAs [J].

Carmi, N ;

Shultz, LA ;

Breaker, RR .

CHEMISTRY & BIOLOGY, 1996, 3 (12) :1039-1046

[6] Characterization of a DNA-cleaving deoxyribozyme [J].

Carmi, N ;

Breaker, RR .

BIOORGANIC & MEDICINAL CHEMISTRY, 2001, 9 (10) :2589-2600

[7] CONSTRUCTION OF BIOLOGICALLY FUNCTIONAL BACTERIAL PLASMIDS IN-VITRO [J].

COHEN, SN ;

CHANG, ACY ;

BOYER, HW ;

HELLING, RB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (11) :3240-3244

[8] A DNA METALLOENZYME WITH DNA-LIGASE ACTIVITY [J].

CUENOUD, B ;

SZOSTAK, JW .

NATURE, 1995, 375 (6532) :611-614

[9] Pseudohexagonal 2D DNA crystals from double crossover cohesion [J].

Ding, BQ ;

Sha, RJ ;

Seeman, NC .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (33) :10230-10231

[10] DNA DOUBLE-CROSSOVER MOLECULES [J].

FU, TJ ;

SEEMAN, NC .

BIOCHEMISTRY, 1993, 32 (13) :3211-3220