Effects of fullerenes and single-wall carbon nanotubes on murine and human macrophages

The discovery in 1985 of C-fullerenes, a novel carbon allotrope with a polygonal structure made up solely by 60 carbon atoms, and in 1991 of C-nanotubes, thin carbon filaments (1-3 mu m in length and 0.001 mu m in diameter) with extraordinary mechanical properties, opened a wide field of activity in carbon research. While toxicity and biocompatibility of C-fullerenes have been widely investigated, literature data concerning the biological properties and biotoxicity of C-nanotubes are poor and contradictory. Here we test the ability of highly purified C-Single-Walled-Nanotubes (SWNTs) and C-fullerenes to elicit an inflammatory response by murine and human macrophage cells in vitro. In order to determine the potential of these C-derivatives as biological inducers of inflammatory reactions we evaluate the ability of C-single-walled nanotubes and C-fullerenes to induce the release of NO by murine macrophages cells, to stimulate the phagocytic activity of human macrophage cells and to be cytotoxic against these cells. We show that SWNTs-C-nanotubes, when highly purified, as well as C-fullerenes, do not stimulate the release of NO by murine macrophage cells in culture, their uptake by human macrophage cells is very low, and they possess a very low toxicity against human macrophage cells. (c) 2005 Elsevier Ltd. All rights reserved.