ELABELA: A Hormone Essential for Heart Development Signals via the Apelin Receptor

被引：399

作者：

Chng, Serene C. ^{[1
]}

Ho, Lena ^{[1
]}

Tian, Jing ^{[1
]}

Reversade, Bruno ^{[1
,2
,3
]}

机构：

[1] ASTAR, Inst Med Biol, Human Genet & Embryol Lab, Singapore 138648, Singapore

[2] ASTAR, Inst Mol & Cellular Biol, Singapore 138673, Singapore

[3] Natl Univ Singapore, Dept Pediat, Singapore 119260, Singapore

来源：

DEVELOPMENTAL CELL | 2013年 / 27卷 / 06期

关键词：

EMBRYONIC STEM-CELLS; ZEBRAFISH; PEPTIDES; APJ; CONTRACTILITY; DEFICIENCY; NARCOLEPSY; GHRELIN; PATHWAY; FIELD;

D O I：

10.1016/j.devcel.2013.11.002

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We report here the discovery and characterization of a gene, ELABELA (ELA), encoding a conserved hormone of 32 amino acids. Present in human embryonic stem cells, ELA is expressed at the onset of zebrafish zygotic transcription and is ubiquitous in the naive ectodermal cells of the embryo. Using zinc-finger-nuclease-mediated gene inactivation in zebrafish, we created an allelic series of ela mutants. ela null embryos have impaired endoderm differentiation potential marked by reduced gata5 and sox17 expression. Loss of Ela causes embryos to develop with a rudimentary heart or no heart at all, surprisingly phenocopying the loss of the apelin receptor (aplnr), which we show serves as Ela's cognate G protein-coupled receptor. Our results reveal the existence of a peptide hormone, ELA, which, together with APLNR, forms an essential signaling axis for early cardiovascular development.

引用

页码：672 / 680

页数：9

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