Involvement of Ca2+ in the Inhibition by Crocetin of Platelet Activity and Thrombosis Formation

被引：18

作者：

Yang, Lina ^{[1
]}

Qian, Zhiyu ^{[1
]}

Yang, Yun ^{[1
]}

Sheng, Liang ^{[1
]}

Ji, Hui ^{[1
]}

Zhou, Chenghua ^{[1
]}

Kazi, Hamid Ali ^{[1
]}

机构：

[1] China Pharmaceut Univ, Dept Pharmacol, Nanjing 210009, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2008年 / 56卷 / 20期

关键词：

Crocetin; platelet aggregation; dense granule release; thrombosis formation; cyclic AMP; Ca2+;

D O I：

10.1021/jf802027a

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Crocetin, a unique carotenoid with potent antioxidative and anti-inflammatory activities, is a major ingredient of saffron used as an important spice and food colorant in various parts of the world. In the present study, the effects of crocetin on platelet activity and thrombosis formation were systematically investigated. Crocetin showed a dose-dependent inhibition of platelet aggregation induced by ADP, collagen, but not by arachidonic acid (AA). Crocetin significantly attenuated dense granule release, while neither platelets adhesion to collagen nor cyclic AMP level was altered by crocetin. Pretreatment with crocetin was confirmed to partially inhibit Ca2+ mobilization via reducing both intracellular Ca2+ release and extracellular Ca2+ influx. Besides that, crocetin prolonged the occlusive time in electrical stimulation-induced carotid arterial thrombosis. These findings suggest that the favorable impacts of crocetin on platelet activity and thrombosis formation may be related to the inhibition of Ca2+ elevation in stimulated platelets.

引用

页码：9429 / 9433

页数：5

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