Utilization of DC-SIGN for Entry of Feline Coronaviruses into Host Cells
被引:43
作者:
Regan, Andrew D.
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机构:
Cornell Univ, Coll Vet Med, Grad Field Microbiol, Ithaca, NY 14853 USACornell Univ, Dept Microbiol & Immunol, Vet Med Ctr C4127, Coll Vet Med, Ithaca, NY 14853 USA
Regan, Andrew D.
[2
]
Whittaker, Gary R.
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机构:
Cornell Univ, Dept Microbiol & Immunol, Vet Med Ctr C4127, Coll Vet Med, Ithaca, NY 14853 USACornell Univ, Dept Microbiol & Immunol, Vet Med Ctr C4127, Coll Vet Med, Ithaca, NY 14853 USA
Whittaker, Gary R.
[1
]
机构:
[1] Cornell Univ, Dept Microbiol & Immunol, Vet Med Ctr C4127, Coll Vet Med, Ithaca, NY 14853 USA
[2] Cornell Univ, Coll Vet Med, Grad Field Microbiol, Ithaca, NY 14853 USA
The entry and dissemination of viruses in several families can be mediated by C-type lectins such as DC-SIGN. We showed that entry of the serotype II feline coronavirus strains feline infectious peritonitis virus FIPV) WSU 79-1146 and DF2 into nonpermissive mouse 3T3 cells can be rescued by the expression of human DC-SIGN hDC-SIGN) and that infection of a permissive feline cell line Crandall-Reese feline kidney) was markedly enhanced by the overexpression of hDC-SIGN. Treatment with mannan considerably reduced infection of feline monocyte-derived cells expressing DC-SIGN, indicating a role for FIPV infection in vivo.