Selective release, removal, and reconstitution of bacteriochlorophyll a molecules into the B800 sites of LH2 complexes from Rhodopseudomonas acidophila 10050

被引：59

作者：

Fraser, NJ

Dominy, PJ

Ücker, B

Simonin, I

Scheer, H

Cogdell, RJ ^{[1
]}

机构：

[1] Univ Glasgow, IBLS, Div Biochem & Mol Biol, Glasgow G12 8QQ, Lanark, Scotland

[2] Univ Munich, Inst Bot, D-80638 Munich, Germany

来源：

BIOCHEMISTRY | 1999年 / 38卷 / 30期

关键词：

D O I：

10.1021/bi990796i

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described which allows the selective release and removal of the Bchla-B800 molecules from the LH2 complex of Rhodopseudomonas acidophila 10050. This procedure also allows reconstitution of similar to 80% of the empty binding sites with native Bchla. As shown by circular dichroism spectroscopy, the overall structures of the B850-only and reconstituted complexes are not affected by the pigment-exchange procedure. The pigments reconstituted into the B800 sites can also efficiently transfer excitation energy to the Bchla-B850 molecules.

引用

收藏

页码：9684 / 9692

页数：9

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