Transient homologous chromosome pairing marks the onset of X inactivation

被引：303

作者：

Xu, N ^{[1
]}

Tsai, CL ^{[1
]}

Lee, JT ^{[1
]}

机构：

[1] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Howard Hughes Med Inst,Dept Mol Biol,Dept Genet, Boston, MA 02114 USA

来源：

SCIENCE | 2006年 / 311卷 / 5764期

关键词：

D O I：

10.1126/science.1122984

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mammalian X inactivation turns off one female X chromosome to enact dosage compensation between XX and XY individuals. X inactivation is known to be regulated in cis by Xite, Tsix, and Xist, but in principle the two Xs must also be regulated in trans to ensure mutually exclusive silencing. Here, we demonstrate that interchromosomal pairing mediates this communication. Pairing occurs transiently at the onset of X inactivation and is specific to the X-inactivation center. Deleting Xite and Tsix perturbs pairing and counting/choice, whereas their autosomal insertion induces de novo X-autosome pairing. Ectopic X-autosome interactions inhibit endogenous X-X pairing and block the initiation of X-chromosome inactivation. Thus, Tsix and Xite function both in cis and in trans. We propose that Tsix and Xite regulate counting and mutually exclusive choice through X-X pairing.

引用

页码：1149 / 1152

页数：4

共 21 条

[1] X-chromosome inactivation: Counting, choice and initiation
Avner, P
Heard, E
[J]. NATURE REVIEWS GENETICS, 2001, 2 (01) : 59 - 67
[2] THE PRODUCT OF THE MOUSE XIST GENE IS A 15 KB INACTIVE X-SPECIFIC TRANSCRIPT CONTAINING NO CONSERVED ORF AND LOCATED IN THE NUCLEUS
BROCKDORFF, N
ASHWORTH, A
KAY, GF
MCCABE, VM
NORRIS, DP
COOPER, PJ
SWIFT, S
RASTAN, S
[J]. CELL, 1992, 71 (03) : 515 - 526
[3] Brown S W, 1992, Seizure, V1, P71, DOI 10.1016/1059-1311(92)90002-I
[4] XIST RNA paints the inactive X chromosome at interphase: Evidence for a novel RNA involved in nuclear chromosome structure
Clemson, CM
McNeil, JA
Willard, HF
Lawrence, JB
[J]. JOURNAL OF CELL BIOLOGY, 1996, 132 (03) : 259 - 275
[5] Capturing chromosome conformation
Dekker, J
Rippe, K
Dekker, M
Kleckner, N
[J]. SCIENCE, 2002, 295 (5558) : 1306 - 1311
[6] Delving into the diversity of facultative heterochromatin: the epigenetics of the inactive X chromosome
Heard, E
[J]. CURRENT OPINION IN GENETICS & DEVELOPMENT, 2005, 15 (05) : 482 - 489
[7] Homologous association of oppositely imprinted chromosomal domains
LaSalle, JM
Lalande, M
[J]. SCIENCE, 1996, 272 (5262) : 725 - 728
[8] Disruption of imprinted X inactivation by parent-of-origin effects at Tsix
Lee, JT
[J]. CELL, 2000, 103 (01) : 17 - 27
[9] Regulation of X-chromosome counting by Tsix and Xite sequences
Lee, JT
[J]. SCIENCE, 2005, 309 (5735) : 768 - 771
[10] Genetic analysis of the mouse X inactivation center defines an 80-kb multifunction domain
Lee, JT
Lu, NF
Han, Y
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (07) : 3836 - 3841

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