Isolation of the lignan secoisolariciresinol diglucoside from flaxseed (Linum usitatissimum L.) by high-speed counter-current chromatography

被引：49

作者：

Degenhardt, A ^{[1
]}

Habben, S ^{[1
]}

Winterhalter, P ^{[1
]}

机构：

[1] Tech Univ Carolo Wilhelmina Braunschweig, Inst Food Chem, DE-38106 Braunschweig, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 943卷 / 02期

关键词：

Linum usitatissimum; counter-current chromatography; plant materials; secoisolariciresinol diglucoside; glucosides; lignans phytoestrogens; plant hormones;

D O I：

10.1016/S0021-9673(01)01467-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-speed counter-current chromatography was successfully used for the isolation and purification of secoisolariciresinol diglucoside, a bioactive lignan from flaxseed (Linum usitatissimum L.). The solvent system consisted of tert.-butylmethyl ether-n-butanol-acetonitrile-water (1:3:1:5). The purity and identity of the isolated compound was checked by high-performance liquid chromatography analysis in combination with mass spectrometry and NMR measurements. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：299 / 302

页数：4

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