Synergistic effects of curcumin with emodin against the proliferation and invasion of breast cancer cells through upregulation of miR-34a

被引：116

作者：

Guo, Jiaoli ^{[1
,2
]}

Li, Wenping ^{[3
]}

Shi, Hongliu ^{[2
]}

Xie, Xinhua ^{[1
,2
]}

Li, Laisheng ^{[4
]}

Tang, Hailin ^{[1
,2
]}

Wu, Minqing ^{[1
,2
]}

Kong, Yanan ^{[1
,2
]}

Yang, Lu ^{[1
,2
]}

Gao, Jie ^{[1
,2
]}

Liu, Peng ^{[1
]}

Wei, Weidong ^{[1
]}

Xie, Xiaoming ^{[1
,2
]}

机构：

[1] Sun Yat Sen Univ, Dept Breast Oncol, Ctr Canc, Guangzhou 510060, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, State Key Lab Oncol South China, Ctr Canc, Guangzhou 510060, Guangdong, Peoples R China

[3] Guangdong Women & Children Hosp, Dept Breast Dis, Maternal & Children Metab Genet Dis Key Lab, Guangzhou 510623, Guangdong, Peoples R China

[4] Sun Yat Sen Univ, Dept Lab Med, Affiliated Hosp 1, Guangzhou 510080, Guangdong, Peoples R China

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 2013年 / 382卷 / 1-2期

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

Curcumin; Emodin; Synergism; miR-34a; Breast cancer; INDUCED APOPTOSIS; EXPRESSION; METASTASIS; GROWTH; COMBINATION; PACLITAXEL; PROGRESSION; SENSITIZES; RESISTANCE; INHIBITORS;

D O I：

10.1007/s11010-013-1723-6

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Curcumin, a biphenyl compound derived from rhizome, is a powerful anti-cancer agent. Emodin is an active component isolated from the root and rhizome of Rheum palmatum that has been widely used in traditional Chinese medicine for the treatment of various diseases. Currently, there are no studies examining the effect of curcumin in combination with emodin on tumor cell growth. In this study, we report for the first time that combined curcumin and emodin administration synergistically inhibits proliferation (MTT assay), survival (flow cytometry), and invasion (transwell migration assay) of breast cancer cells. Synergism is determined by the Chou-Talalay method. Moreover, we demonstrate that miR-34a is upregulated by curcumin and emodin. This microRNA helps mediate the anti-tumor effects of curcumin and emodin by downregulating Bcl-2 and Bmi-1. Our results not only provide insight into the mechanism of synergy between curcumin and emodin in breast cancer cells, but also suggest a new and potentially useful approach for breast cancer therapy.

引用

页码：103 / 111

页数：9

共 49 条

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