Human receptors for sweet and umami taste

被引:1041
作者
Li, XD
Staszewski, L
Xu, H
Durick, K
Zoller, M
Adler, E
机构
[1] Senomyx Inc, La Jolla, CA 92037 USA
[2] Aurora Biosci Corp, San Diego, CA 92121 USA
关键词
D O I
10.1073/pnas.072090199
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The three members of the T1R class of taste-specific G protein-coupled receptors have been hypothesized to function in combination as heterodimeric sweet taste receptors. Here we show that human T1R2/T1R3 recognizes diverse natural and synthetic sweeteners. In contrast, human T1R1/T1R3 responds to the umami taste stimulus L-glutamate, and this response is enhanced by 5'-ribonucleotides, a hallmark of umami taste. The ligand specificities of rat T1R2/T1R3 and T1R1/T1R3 correspond to those of their human counterparts. These findings implicate the T1Rs in umami taste and suggest that sweet and umami taste receptors share a common subunit.
引用
收藏
页码:4692 / 4696
页数:5
相关论文
共 28 条
  • [1] Positional cloning of the mouse saccharin preference (Sac) locus
    Bachmanov, AA
    Li, X
    Reed, DR
    Ohmen, JD
    Li, SR
    Chen, ZY
    Tordoff, MG
    de Jong, PJ
    Wu, CY
    West, DB
    Chatterjee, A
    Ross, DA
    Beauchamp, GK
    [J]. CHEMICAL SENSES, 2001, 26 (07) : 925 - 933
  • [2] ELECTROPHYSIOLOGICAL STUDY OF GUSTATORY EFFECTS OF SWEET PROTEINS MONELLIN AND THAUMATIN IN MONKEY, GUINEA-PIG AND RAT
    BROUWER, JN
    HELLEKANT, G
    KASAHARA, Y
    VANDERWE.H
    ZOTTERMAN, Y
    [J]. ACTA PHYSIOLOGICA SCANDINAVICA, 1973, 89 (04): : 550 - 557
  • [3] T2Rs function as bitter taste receptors
    Chandrashekar, J
    Mueller, KL
    Hoon, MA
    Adler, E
    Feng, LX
    Guo, W
    Zuker, CS
    Ryba, NJP
    [J]. CELL, 2000, 100 (06) : 703 - 711
  • [4] A metabotropic glutamate receptor variant functions as a taste receptor
    Chaudhari, N
    Landin, MA
    Roper, SD
    [J]. NATURE NEUROSCIENCE, 2000, 3 (02) : 113 - 119
  • [5] Chaudhari N, 1996, J NEUROSCI, V16, P3817
  • [6] SUBSTITUTION OF 3 AMINO-ACIDS SWITCHES RECEPTOR SPECIFICITY OF G(Q)ALPHA TO THAT OF G(I)ALPHA
    CONKLIN, BR
    FARFEL, Z
    LUSTIG, KD
    JULIUS, D
    BOURNE, HR
    [J]. NATURE, 1993, 363 (6426) : 274 - 276
  • [7] Taste preference synergy between glutamate receptor agonists and inosine monophosphate in rats
    Delay, ER
    Beaver, AJ
    Wagner, KA
    Stapleton, JR
    Harbaugh, JO
    Catron, KD
    Roper, SD
    [J]. CHEMICAL SENSES, 2000, 25 (05) : 507 - 515
  • [8] SINGLE-LOCUS CONTROL OF SACCHARIN PREFERENCE IN MICE
    FULLER, JL
    [J]. JOURNAL OF HEREDITY, 1974, 65 (01) : 33 - 36
  • [9] Putative mammalian taste receptors: A class of taste-specific GPCRs with distinct topographic selectivity
    Hoon, MA
    Adler, E
    Lindemeier, J
    Battey, JF
    Ryba, NJP
    Zuker, CS
    [J]. CELL, 1999, 96 (04) : 541 - 551
  • [10] GABAB receptors function as a heteromeric assembly of the subunits GABABR1 and GABABR2
    Jones, KA
    Borowsky, B
    Tamm, JA
    Craig, DA
    Durkin, MM
    Dai, M
    Yao, WJ
    Johnson, M
    Gunwaldsen, C
    Huang, LY
    Tang, C
    Shen, QR
    Salon, JA
    Morse, K
    Laz, T
    Smith, KE
    Nagarathnam, D
    Noble, SA
    Branchek, TA
    Gerald, C
    [J]. NATURE, 1998, 396 (6712) : 674 - 679