Functional analysis of pneumolysin by use of monoclonal antibodies

被引：58

作者：

delosToyos, JR

Mendez, FJ

Aparicio, JF

Vazquez, F

Suarez, MD

Fleites, A

Hardisson, C

Morgan, PJ

Andrew, PW

Mitchell, TJ

机构：

[1] UNIV LEICESTER,DEPT MICROBIOL & IMMUNOL,LEICESTER LE1 9HN,LEICS,ENGLAND

[2] UNIV OVIEDO,FAC MED,AREA MICROBIOL,E-33006 OVIEDO,SPAIN

来源：

INFECTION AND IMMUNITY | 1996年 / 64卷 / 02期

关键词：

D O I：

10.1128/IAI.64.2.480-484.1996

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have produced a panel of monoclonal antibodies to pneumolysin, the membrane-damaging toxin from Streptococcus pneumoniae. We have used these antibodies to identify three regions of the toxin sequence that are involved in the lytic mechanism of this toxin, Two of these sites probably form the cell binding site of this toxin. Antibodies to the third site inhibit the lytic action of this toxin but not the binding of this toxin to cells. This site is engaged in the oligomerization process involved in the formation of pores in cell membranes. Two of these epitopes are also present in the related toxin perfringolysin O.

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页码：480 / 484

页数：5

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