The stress response of the gilthead sea bream (Sparus aurata L.) to air exposure and confinement

被引:275
作者
Arends, RJ
Mancera, JM
Muñoz, JL
Bonga, SEW
Flik, G
机构
[1] Univ Nijmegen, Dept Anim Physiol, NL-6525 ED Nijmegen, Netherlands
[2] Univ Cadiz, Fac Ciencias Mar, Dept Biol Anim, Cadiz 11510, Spain
[3] CICEM El Toruno, Cadiz 11510, Spain
关键词
D O I
10.1677/joe.0.1630149
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
We investigated short-term effects (up to 24 h) of air exposure and confinement, and long-term effects (up to 11 days) of confinement, to elucidate signalling pathways in the stress response of gilthead sea bream Sparus aurata L. Plasma glucose and lactate were taken as indicators of sympathetic activation, and alpha-melanocyte stimulating hormone (alpha-MSH), adrenocorticotrophic hormone (ACTH) and cortisol as indicators of activation of the brain-pituitary- interrenal (BPI) axis. Air exposure for 3 min resulted, within 30 min, in an increase in plasma concentrations of cortisol, alpha-MSH, glucose, lactate, osmolality and plasma Na, Cl and Mg. Plasma ACTH and beta-endorphin and plasma K, Ca and P did not change. We conclude that air exposure mainly activates the brain-sympathetic-chromaffin cell. (BSC) axis. In fish confined at a density of 70 kg/m(3) (compared with 4 kg/m3 in controls), cortisol, ACTH and alpha-MSH increased within 1 h, indicating activation of the BPI axis. Plasma glucose, Na, Cl and Mg increased with an 8 h delay compared with the response to air exposure. No changes in plasma lactate, osmolality, K, Ca and P were observed. Long-term confinement induced a biphasic cortisol response with peaks at 1 h and at 2 and 3 days. A gradual increase in plasma beta-endorphin concentrations peaked at 7 days; the concentration of alpha-MSH increased rapidly within 1 h and then declined to control values 4 days after the onset of confinement. No changes in ACTH were detected. Our data provide evidence that a stresser-specific activation of the BSC and BPI axes may occur in Sparus aurata.
引用
收藏
页码:149 / 157
页数:9
相关论文
共 45 条
[1]   PHYSIOLOGICAL-RESPONSES OF THE GILTHEAD SEA BREAM SPARUS-AURATA TO HYPOOSMOTIC SHOCK [J].
ALTIMIRAS, J ;
CHAMPION, SR ;
PUIGCERVER, M ;
TORT, L .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY, 1994, 108 (01) :81-85
[2]  
[Anonymous], FISH PHYSL B
[3]   ENDORPHIN AND MSH IN CONCERT FORM THE CORTICOTROPIC PRINCIPLE RELEASED BY TILAPIA (OREOCHROMIS-MOSSAMBICUS, TELEOSTEI) MELANOTROPES [J].
BALM, PHM ;
HOVENS, MLM ;
BONGA, SEW .
PEPTIDES, 1995, 16 (03) :463-469
[4]   ADRENOCORTICOTROPIC HORMONE IN RELATION TO INTERRENAL FUNCTION DURING STRESS IN TILAPIA (OREOCHROMIS-MOSSAMBICUS) [J].
BALM, PHM ;
PEPELS, P ;
HELFRICH, S ;
HOVENS, MLM ;
BONGA, SEW .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1994, 96 (03) :347-360
[5]  
Barton B.A., 1991, Annual Review of Fish Diseases, V1, P3, DOI 10.1016/0959-8030(91)90019-G
[6]  
BARTON BA, 1987, DIS AQUAT ORGAN, V2, P173
[7]   The stress response in fish [J].
Bonga, SEW .
PHYSIOLOGICAL REVIEWS, 1997, 77 (03) :591-625
[8]   Time-course studies on plasma glucose, insulin, and cortisol in sea bass (Dicentrarchus labrax) held under different photoperiodic regimes [J].
Cerdá-Reverter, JM ;
Zanuy, S ;
Carrillo, M ;
Madrid, JA .
PHYSIOLOGY & BEHAVIOR, 1998, 64 (03) :245-250
[9]   The response to capture and confinement stress of plasma cortisol, plasma sex steroids and vitellogenic oocytes in the marine teleost, red gurnard [J].
Clearwater, SJ ;
Pankhurst, NW .
JOURNAL OF FISH BIOLOGY, 1997, 50 (02) :429-441
[10]   PROTEOLYTIC CLEAVAGE OF ACTH IN CORTICOTROPES OF SEXUALLY MATURE AXOLOTLS (AMBYSTOMA-MEXICANUM) [J].
DORES, RM ;
SANDOVAL, FL ;
MCDONALD, LK .
PEPTIDES, 1993, 14 (05) :1029-1035