Frequency and stability of chromosomal integration of adenovirus vectors

被引:157
作者
Harui, A
Suzuki, S
Kochanek, S
Mitani, K
机构
[1] Univ Calif Los Angeles, Sch Med, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USA
[2] Jonsson Comprehens Canc Ctr, Los Angeles, CA 90095 USA
[3] Univ Cologne, ZMMK, Ctr Mol Med, D-50931 Cologne, Germany
关键词
D O I
10.1128/JVI.73.7.6141-6146.1999
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
One of the limitations of adenovirus vectors is the lack of machinery necessary for their integration into host chromosomes, resulting in short-term gene expression in dividing cells. We analyzed frequencies of integration and persistence of gene expression from integrated adenovirus vectors. Both El-substituted and helper-dependent adenovirus vectors achieved similar integration efficiencies of similar to 10(-3) to 10(-5) per cell, with the helper-dependent vector showing slightly higher efficiencies. Un stable cell pools, gene expression of the integrated vector persisted for at least 50 cell divisions without selection. However, some stable cell clones showed changes in gene expression, which were accompanied by structural changes in the integrated vector DNA.
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收藏
页码:6141 / 6146
页数:6
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