Increased sensitivity to peroxidative agents as a possible pathogenic factor of melanocyte damage in vitiligo

被引:215
作者
Maresca, V
Roccella, M
Roccella, F
Camera, E
DelPorto, G
Passi, S
Grammatico, P
Picardo, M
机构
[1] SAN GALLICANO DERMATOL INST,I-00153 ROME,ITALY
[2] UNIV ROMA LA SAPIENZA,CHAIR MED GENET,ROME,ITALY
[3] IST DERMOPAT IMMACOLATA,ROME,ITALY
关键词
free radicals; superoxide dismutase; catalase; vitamin E; ubiquinone; oxidative stress;
D O I
10.1111/1523-1747.ep12335801
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
To examine the sensitivity of vitiligo melanocytes to external oxidative stress, we studied enzymatic and non-enzymatic anti-oxidants in cultured melanocytes of normal subjects (n = 20) and melanocytes from apparently normal skin of vitiligo patients (n = 10). The activity of superoxide dismutase and catalase and the intracellular concentrations of vitamin E and ubiquinone were evaluated in cultures at the fourth or fifth passage, In addition, cells were exposed to various concentrations of a peroxidizing agent, cumene hydroperoxide (CUH, 0.66-20 mu M), for 1 and 24 h, Compared to normal melanocytes, vitiligo melanocytes showed normal superoxide dismutase and significantly lower catalase activities and higher vitamin E and lower ubiquinone levels. At the concentration used, CUH did not significantly affect cell number or viability of melanocytes after either period of culture, On the contrary, vitiligo melanocytes were susceptible to the toxic effect of CUH after 24 h of continuous treatment at concentrations greater than 6.6 mu M. The degree of CUH toxicity correlated strictly with the anti-oxidant pattern, defined as the ratio between vitamin E concentration and catalase activity, suggesting chat the alteration in the antioxidants was the basis for sensitivity to the external oxidative stress, Our results demonstrate the presence of an imbalance in the anti-oxidant system in vitiligo melanocytes and provide further support for a free radical-mediated damage as an initial pathogenic event in melanocyte degeneration in vitiligo.
引用
收藏
页码:310 / 313
页数:4
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