Molecular cloning of prohormone convertase 1 from the atrial gland of Aplysia

被引：19

作者：

Gorham, EL

Nagle, GT

Smith, JS

Shen, H

Kurosky, A

机构：

[1] UNIV TEXAS,MED BRANCH,DEPT HUMAN BIOL CHEM & GENET,GALVESTON,TX 77555

[2] UNIV TEXAS,MED BRANCH,INST MARINE BIOMED,GALVESTON,TX 77555

[3] UNIV TEXAS,MED BRANCH,DEPT ANAT & NEUROSCI,GALVESTON,TX 77555

来源：

DNA AND CELL BIOLOGY | 1996年 / 15卷 / 04期

关键词：

D O I：

10.1089/dna.1996.15.339

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have screened an Aplysia atrial gland cDNA library using a prohormone convertase (PC)1 probe prepared by polymerase chain reaction (PCR) and have isolated an Aplysia PC1-related full-length 3.6-kb cDNA clone. The cDNA sequence (3,565 bp) encoded a putative preproendoprotease (APC1) of 703 amino acid residues that showed considerable sequence identity with other eukaryotic PC1s, and indicated a high degree of sequence identity with an Aplysia nervous system PC sequence (aPC1B). Northern blot analysis of atrial gland RNA identified two APC1 transcripts of 3.9 kb and 5.0 kb. APC1 is a candidate PC that may play an important role in the processing of egg-laying hormone (ELH)-related precursors in atrial gland secretory cells and represents one of the first examples of PC1 expression in an exocrine tissue.

引用

收藏

页码：339 / 345

页数：7

相关论文

共 34 条

[1] A MEMBER OF THE EUKARYOTIC SUBTILISIN FAMILY (PC3) HAS THE ENZYMATIC-PROPERTIES OF THE TYPE-1 PROINSULIN-CONVERTING ENDOPEPTIDASE [J].

BAILYES, EM ;

SHENNAN, KIJ ;

SEAL, AJ ;

SMEEKENS, SP ;

STEINER, DF ;

HUTTON, JC ;

DOCHERTY, K .

BIOCHEMICAL JOURNAL, 1992, 285 :391-394

[2] PC1 AND PC2 ARE PROPROTEIN CONVERTASES CAPABLE OF CLEAVING PROOPIOMELANOCORTIN AT DISTINCT PAIRS OF BASIC RESIDUES [J].

BENJANNET, S ;

RONDEAU, N ;

DAY, R ;

CHRETIEN, M ;

SEIDAH, NG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (09) :3564-3568

[3] PROHORMONE-CONVERTING ENZYMES - REGULATION AND EVALUATION OF FUNCTION USING ANTISENSE RNA [J].

BLOOMQUIST, BT ;

EIPPER, BA ;

MAINS, RE .

MOLECULAR ENDOCRINOLOGY, 1991, 5 (12) :2014-2024

[4] THE EUKARYOTIC PROHORMONE-PROCESSING ENDOPROTEASES [J].

BLOOMQUIST, BT ;

MAINS, RE .

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 1993, 3 (3-4) :197-212

[5] CONSERVATION OF THE PROHORMONE CONVERTASE GENE FAMILY IN METAZOA - ANALYSIS OF CDNAS ENCODING A PC3-LIKE PROTEIN FROM HYDRA [J].

CHAN, SJ ;

OLIVA, AA ;

LAMENDOLA, J ;

GRENS, A ;

BODE, H ;

STEINER, DF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (15) :6678-6682

[6] THE FUNCTION AND DIFFERENTIAL SORTING OF A FAMILY OF APLYSIA PROHORMONE PROCESSING ENZYMES [J].

CHUN, JY ;

KORNER, J ;

KREINER, T ;

SCHELLER, RH ;

AXEL, R .

NEURON, 1994, 12 (04) :831-844

[7]

DELIDOW BC, 1993, PCR PROTOCOLS CURREN, V15, P1

[8] A NOVEL ACTIN CDNA IS EXPRESSED IN THE NEURONS OF APLYSIA-CALIFORNICA [J].

DESGROSEILLERS, L ;

AUCLAIR, D ;

WICKHAM, L ;

MAALOUF, M .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1994, 1217 (03) :322-324

[9] GENE ORGANIZATION OF THE MOUSE PROHORMONE AND PRO-PROTEIN CONVERTASE PC1 [J].

FTOUHI, N ;

DAY, R ;

MBIKAY, M ;

CHRETIEN, M ;

SEIDAH, NG .

DNA AND CELL BIOLOGY, 1994, 13 (04) :395-407

[10] YEAST PROHORMONE PROCESSING ENZYME (KEX2 GENE-PRODUCT) IS A CA-2+-DEPENDENT SERINE PROTEASE [J].

FULLER, RS ;

BRAKE, A ;

THORNER, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (05) :1434-1438

← 1 2 3 4 →