A novel test for identifying genes involved in aldehyde detoxification in the yeast. Increased sensitivity of superoxide-deficient yeast to aldehydes and their metabolic precursors

被引：9

作者：

Bilinski, T

Kwolek, M

Sas, E

Krynicka, M

Koziol, S

Owsiak-Teleon, A

Krzepilko, A

Bartosz, G

机构：

[1] Univ Rzeszow, Dept Biochem & Cell Biol, PL-35959 Rzeszow, Poland

[2] Zamosc Coll Agr, Dept Biochem, Zamosc, Poland

来源：

BIOFACTORS | 2005年 / 24卷 / 1-4期

关键词：

yeast; Saccharomyces cerevisiae; aldehydes; acrolein; allyl alcohol; superoxide dismutase;

D O I：

10.1002/biof.5520240107

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel test for the identification of genes involved in aldehyde metabolism is proposed, based on detection of altered sensitivity of the yeast to corresponding alcohols, metabolic precursors of the aldehydes. This attitude enabled to an unexpected detection increased sensitivity of mutants devoid of CuZn-superoxide dismutase (CuZnSOD) to allyl alcohol ( precursor of acrolein) and nonenol. We interpret this finding as due to inactivation of some important element of aldehyde detoxification by increased flux of superoxide in Delta CuZnSOD mutants.

引用

页码：59 / 65

页数：7

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