Visualization of in vitro protein-rejecting properties of PEGylated stealth® polycyanoacrylate nanoparticles

被引：157

作者：

Peracchia, MT

Harnisch, S

Pinto-Alphandary, H

Gulik, A

Dedieu, JC

Desmaële, D

d'Angelo, J

Müller, RH

Couvreur, P

机构：

[1] Univ Paris 11, CNRS, URA Physicochim 1218, F-92296 Chatenay Malabry, France

[2] Free Univ Berlin, Inst Pharm, D-1216 Berlin, Germany

[3] CNRS, Ctr Genet Mol, UPR A2420, F-91198 Gif Sur Yvette, France

[4] Univ Paris 11, CNRS, URA Chim Organ 1843, F-92296 Chatenay Malabry, France

来源：

BIOMATERIALS | 1999年 / 20卷 / 14期

关键词：

nanoparticles; stealth; protein adsorption; polycyanoacrylate; PEG;

D O I：

10.1016/S0142-9612(99)00021-6

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The in vitro protein-rejecting properties of PEG-coated polyalkylcyanoacrylate (PACA) nanoparticles were for the first time visualized after freeze-fracture of the nanoparticles pre-incubated with fibrinogen as a model blood protein. The reduced protein association to the nanoparticles was evidenced also by two-dimensional PAGE after incubation of the nanoparticles with human plasma. In vivo experiments showed the 'stealth' long-circulating properties of the PEGylated nanoparticles after intravenous administration to mice. Thus, the images obtained after nanoparticle-protein incubation were predictive of the behavior observed in vivo. In conclusion, freeze-fracture analysis represents a novel and original qualitative approach. to investigate the interactions between proteins and particulate systems. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：1269 / 1275

页数：7

共 23 条

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