Molecular cloning and functional characterization of two alternatively spliced Ntcp isoforms from mouse liver

被引：45

作者：

Cattori, V

Eckhardt, U

Hagenbuch, B ^{[1
]}

机构：

[1] Univ Zurich Hosp, Dept Med, Div Clin Pharmacol & Toxicol, CH-8091 Zurich, Switzerland

[2] ETH Zurich, Inst Biochem, CH-8092 Zurich, Switzerland

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1999年 / 1445卷 / 01期

关键词：

Na+/taurocholate cotransporting polypeptide; Ntcp; bile salt;

D O I：

10.1016/S0167-4781(99)00029-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To isolate the murine Na+/taurocholate cotransporting polypeptide (Ntcp), we screened a mouse liver cDNA library and identified Ntcp1, encoding a 362 amino acid protein and Ntcp2, encoding a 317 amino acid protein which had a shorter C-terminal end. Both isoforms mediated saturable Na+-dependent transport of taurocholate when expressed in Xenopus laevis oocytes. Analysis of the gene revealed that Ntcp2 is produced by alternative splicing where the last intron is retained. (C) 1999 Elsevier Science B.V. All rights reserved.

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页码：154 / 159

页数：6

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