Simple, but not branched, plasmodesmata allow the nonspecific trafficking of proteins in developing tobacco leaves

被引:335
作者
Oparka, KJ [1 ]
Roberts, AG
Boevink, P
Santa Cruz, S
Roberts, L
Pradel, KS
Imlau, A
Kotlizky, G
Sauer, N
Epel, B
机构
[1] Scottish Crop Res Inst, Cell Biol Unit, Dundee DD2 5DA, Scotland
[2] Univ Erlangen Nurnberg, Lehrstuhl Bot 2, D-91058 Erlangen, Germany
[3] Tel Aviv Univ, Dept Plant Sci, IL-69978 Tel Aviv, Israel
关键词
D O I
10.1016/S0092-8674(00)80786-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Leaves undergo a sink-source transition during which a physiological change occurs from carbon import to export. In sink leaves, biolistic bombardment of plasmids encoding GFP-fusion proteins demonstrated that proteins with an M-r up to 50 kDa could move freely through plasmodesmata. During the sink-source transition, the capacity to traffic proteins decreased substantially and was accompanied by a developmental switch from simple to branched forms of plasmodesmata. inoculation of sink leaves with a movement protein-defective virus showed that virally expressed GFP, but not viral RNA, was capable of trafficking between sink cells during infection. Contrary to dogma that plasmodesmata have a size exclusion limit below 1 kDa, the data demonstrate that nonspecific "macromolecular trafficking" is a general feature of simple plasmodesmata in sink leaves.
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页码:743 / 754
页数:12
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