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Measles immunity testing: Comparison of two measles IgG ELISAs with plaque reduction neutralisation assay

被引：70
作者：
Cohen, BJ
Parry, RP
Doblas, D
Samuel, D
Warrener, L
Andrews, N
Brown, D
机构：
[1] Hlth Protect Agcy, Ctr Infect, Virus Ref Dept, London NW9 5HT, England
[2] Hlth Protect Agcy, Ctr Infect, Stat Unit, London NW9 5HT, England
来源：
JOURNAL OF VIROLOGICAL METHODS | 2006年 / 131卷 / 02期
关键词：
measles; immunity; neutralisation; ELISA;
D O I：
10.1016/j.jviromet.2005.08.001
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Two commercial IgG ELISAs, one based on recombinant nucleocapsid antigen and one based on cell culture grown native virus antigens, were evaluated for measles immunity testing by comparison with plaque reduction neutralisation test (PRNT). Qualitative results of the two ELISAs showed 92% agreement with those of PRNT. The sensitivity of the two ELISAs was 89.6%. False negative ELISA results were obtained in 10% of sera, mainly sera containing low levels of neutralising antibody. The specificity of both ELISAs was 100%. Measles IgG ELISAs perform adequately for immunity testing, correctly identifying seronegative individuals for vaccination. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码：209 / 212
页数：4
相关论文
共 11 条
[1]   ROLE OF VIRUS-STRAIN IN CONVENTIONAL AND ENHANCED MEASLES PLAQUE NEUTRALIZATION TEST [J].
ALBRECHT, P ;
HERRMANN, K ;
BURNS, GR .
JOURNAL OF VIROLOGICAL METHODS, 1981, 3 (05) :251-260
[2]   Follow-up of infants given measles vaccine at 6 months of age:: antibody and CMI responses to MMRII® at 15 months of age and antibody levels at 27 months of age [J].
Carson, MM ;
Spady, DW ;
Beeler, JA ;
Krezolek, MP ;
Audet, S ;
Pabst, HF .
VACCINE, 2005, 23 (25) :3247-3255
[3]   MEASLES ANTIBODY - REEVALUATION OF PROTECTIVE TITERS [J].
CHEN, RT ;
MARKOWITZ, LE ;
ALBRECHT, P ;
STEWART, JA ;
MOFENSON, LM ;
PREBLUD, SR ;
ORENSTEIN, WA .
JOURNAL OF INFECTIOUS DISEASES, 1990, 162 (05) :1036-1042
[4]  
CRONIN M, 2000, MEASLES OUTBREAK REP
[5]  
Griffin DE, 2001, MEASLES VIRUS FIELDS, P1401
[6]   An evaluation of nine commercial EIA kits for the detection of measles specific IgG [J].
Hesketh, L ;
Charlett, A ;
Farrington, P ;
Miller, E ;
Forsey, T ;
MorganCapner, P .
JOURNAL OF VIROLOGICAL METHODS, 1997, 66 (01) :51-59
[7]  
MINSHI L, 1999, J VIROL METHODS, V78, P209
[8]   Development of a measles specific IgM ELISA for use with serum and oral fluid samples using recombinant measles nucleoprotein produced in Saccharomyces cerevisiae [J].
Samuel, D ;
Sasnauskas, K ;
Jin, L ;
Gedvilaite, A ;
Slibinskas, R ;
Beard, S ;
Zvirbliene, A ;
Oliveira, SA ;
Staniulis, J ;
Cohen, B ;
Brown, D .
JOURNAL OF CLINICAL VIROLOGY, 2003, 28 (02) :121-129
[9]   FURTHER-ATTENUATED MEASLES-VACCINE - VIRUS PASSAGES AFFECT VIRAL SURFACE PROTEIN EXPRESSION, IMMUNOGENICITY AND HISTOPATHOLOGY PATTERN INVIVO [J].
SINITSYNA, OA ;
KHUDAVERDYAN, OE ;
STEINBERG, LL ;
NAGIEVA, FG ;
LOTTE, VD ;
DOROFEEVA, LV ;
ROZINA, EE ;
BORISKIN, YS .
RESEARCH IN VIROLOGY, 1990, 141 (05) :517-531
[10]  
*WHO, 2005, MEASL
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