Analysis of gene expression in rose petals using expressed sequence tags

被引:66
作者
Channelière, S
Rivière, S
Scalliet, G
Szecsi, J
Jullien, F
Dolle, C
Vergne, P
Dumas, C
Bendahmane, M
Hugueney, P
Cock, JM [1 ]
机构
[1] Ecole Normale Super Lyon, CNRS INRA ENSL, UMR 5667, F-69364 Lyon 07, France
[2] Jardin Bot Lyon, F-69459 Lyon 06, France
[3] Univ Jean Monnet, Lab Biotechnol Vegetales Appl Plantes Aromat & Me, F-42023 St Etienne Du Rouvray 2, France
来源
FEBS LETTERS | 2002年 / 515卷 / 1-3期
关键词
expressed sequence tag; petal; rose; scent; senescence; Rosa chinensis;
D O I
10.1016/S0014-5793(02)02413-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Single-pass sequences were obtained from the 5'-ends of a total of 1794 rose petal cDNA clones. Cluster analysis identified 242 groups of sequences and 635 singletons indicating that the database represents a total of 877 genes. Putative functions could be assigned to 1151 of the transcripts. Expression analysis indicated that transcripts of several of the genes identified accumulated specifically in petals and stamens. The cDNA library and expressed sequence tag database described here represent a valuable resource for future research aimed at improving economically important rose characteristics such as flower form, longevity and scent. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:35 / 38
页数:4
相关论文
共 22 条
  • [1] ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
  • [2] BROCARD I, 1999, THESIS U PARIS SUD
  • [3] Natural antisense transcripts of the S locus receptor kinase gene and related sequences in Brassica oleracea
    Cock, JM
    Swarup, R
    Dumas, C
    [J]. MOLECULAR & GENERAL GENETICS, 1997, 255 (05): : 514 - 524
  • [4] Construction of a genetic linkage map for roses using RAPD and AFLP markers
    Debener, T
    Mattiesch, L
    [J]. THEORETICAL AND APPLIED GENETICS, 1999, 99 (05) : 891 - 899
  • [5] REGENERATION OF TRANSGENIC ROSE (ROSA-HYBRIDA) PLANTS FROM EMBRYOGENIC TISSUE
    FIROOZABADY, E
    MOY, Y
    COURTNEYGUTTERSON, N
    ROBINSON, K
    [J]. BIO-TECHNOLOGY, 1994, 12 (06): : 609 - 613
  • [6] An investigation of the storage and biosynthesis of phenylpropenes in sweet basil
    Gang, DR
    Wang, JH
    Dudareva, N
    Nam, KH
    Simon, JE
    Lewinsohn, E
    Pichersky, E
    [J]. PLANT PHYSIOLOGY, 2001, 125 (02) : 539 - 555
  • [7] CAP3: A DNA sequence assembly program
    Huang, XQ
    Madan, A
    [J]. GENOME RESEARCH, 1999, 9 (09) : 868 - 877
  • [8] KOVATS ES, 1987, J CHROMATOGR, V406, P185
  • [9] Krussmann G, 1982, ROSES
  • [10] Probing essential oil biosynthesis and secretion by functional evaluation of expressed sequence tags from mint glandular trichomes
    Lange, BM
    Wildung, MR
    Stauber, EJ
    Sanchez, C
    Pouchnik, D
    Croteau, R
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (06) : 2934 - 2939