Crystal Structure of the DNA Cytosine Deaminase APOBEC3F: The Catalytically Active and HIV-1 Vif-Binding Domain

被引：88

作者：

Bohn, Markus-Frederik ^{[1
]}

Shandilya, Shivender M. D. ^{[1
]}

Albin, John S. ^{[4
]}

Kouno, Takahide ^{[4
]}

Anderson, Brett D. ^{[4
]}

McDougle, Rebecca M. ^{[4
]}

Carpenter, Michael A. ^{[4
]}

Rathore, Anurag ^{[4
]}

Evans, Leah ^{[4
]}

Davis, Ahkillah N. ^{[4
]}

Zhang, JingYing ^{[4
]}

Lu, Yongjian ^{[4
]}

Somasundaran, Mohan ^{[2
,3
]}

Matsuo, Hiroshi ^{[4
]}

Harris, Reuben S. ^{[4
]}

Schiffer, Celia A. ^{[1
]}

机构：

[1] Univ Massachusetts, Sch Med, Dept Biochem & Mol Pharmacol, Worcester, MA 01605 USA

[2] Univ Massachusetts, Sch Med, Dept Pediat, Worcester, MA 01605 USA

[3] Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA 01605 USA

[4] Univ Minnesota, Dept Biochem Mol Biol & Biophys, Inst Mol Virol, Minneapolis, MN 55455 USA

来源：

STRUCTURE | 2013年 / 21卷 / 06期

关键词：

HUMAN-IMMUNODEFICIENCY-VIRUS; CYTIDINE DEAMINASE; TYPE-1; VIF; FUNCTIONAL IMPLICATIONS; RESTRICTION FACTORS; VIRAL INFECTIVITY; INNATE IMMUNITY; AMINO-ACID; IN-VIVO; PROTEIN;

D O I：

10.1016/j.str.2013.04.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Human APOBEC3F is an antiretroviral single-strand DNA cytosine deaminase, susceptible to degradation by the HIV-1 protein Vif. In this study the crystal structure of the HIV Vif binding, catalytically active, C-terminal domain of APOBEC3F (A3F-CTD) was determined. The A3F-CTD shares structural motifs with portions of APOBEC3G-CTD, APOBEC3C, and APOBEC2. Residues identified to be critical for Vif-dependent degradation of APOBEC3F all fit within a predominantly negatively charged contiguous region on the surface of A3F-CTD. Specific sequence motifs, previously shown to play a role in Vif susceptibility and virion encapsidation, are conserved across APOBEC3s and between APOBEC3s and HIV-1 Vif. In this structure these motifs pack against each other at intermolecular interfaces, providing potential insights both into APOBEC3 oligomerization and Vif interactions.

引用

页码：1042 / 1050

页数：9

共 61 条

[1]

A Single Amino Acid in Human APOBEC3F Alters Susceptibility to HIV-1 Vif [J].