Detection of bradykinin and bradykinin-β2 receptors in the porcine endometrium during the estrous cycle and early pregnancy

被引:11
作者
Allen, MR
Zhang, BR
Hettinger, AM
Goad, DW
Malayer, JR
Geisert, RD
机构
[1] Oklahoma State Univ, Oklahoma Agr Expt Stn, Dept Anim Sci, Stillwater, OK 74078 USA
[2] Oklahoma State Univ, Coll Vet Med, Dept Physiol Sci, Stillwater, OK 74078 USA
关键词
conceptus; female reproductive tract; gene regulation; implantation; uterus;
D O I
10.1095/biolreprod66.3.574
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
During the period of attachment of the trophectoderm to the uterine lumenal surface in the pig, there is an increase in uterine blood flow and a localized hyperemic response induced by the developing conceptuses. The presence of tissue kallikrein in the porcine uterine lumen suggests that the kallikrein-kinin system may be functional during pregnancy in the pig. The objective of the present study was to determine the concentration of bradykinin within the uterine lumen during the estrous cycle and early pregnancy as well as endometrial gene expression and cellular localization of the bradykinin beta(2) receptor. Concentration of bradykinin in uterine flushings was greatest during estrus (Day 0) and Days 12-18 of the estrous cycle. However, there was a 5- to 10-fold increase in bradykinin content in pregnant uterine flushings on Days 12-18 of pregnancy compared with the estrous cycle. Endometrial bradykinin beta(2) receptor gene expression was greatest on Days 0, 12, 15, and 18 of the estrous cycle and pregnancy as gene expression decreased almost 6-fold on Days 5 and 10. Bradykinin beta(2) receptors were detected in the endometrial surface and glandular epithelium with greatest intensity of staining observed on Days 0, 12, 15, and 18 of the estrous cycle and pregnancy. Results from the present study suggest that the kallikrein-kinin system plays a role in the establishment of pregnancy in the pig.
引用
收藏
页码:574 / 579
页数:6
相关论文
共 37 条
[1]  
BAZER FW, 1992, P SOC EXP BIOL MED, V199, P373, DOI 10.3181/00379727-199-43371A
[2]  
BHOOLA KD, 1992, PHARMACOL REV, V44, P1
[3]   CHANGES IN KININOGENS AND KALLIKREIN IN THE PLASMA, BRAIN, AND UTERUS DURING PREGNANCY IN THE RAT [J].
BRANN, DW ;
GREENBAUM, L ;
MAHESH, VB ;
GAO, XX .
ENDOCRINOLOGY, 1995, 136 (01) :46-51
[4]   Temporal and tissue-specific expression of kallikrein (Klk) genes and identification of a novel Klk messenger ribonucleic acid transcript during early development in the mouse [J].
Chan, CS ;
Harvey, MB ;
Clements, JA .
BIOLOGY OF REPRODUCTION, 1999, 61 (03) :621-628
[5]  
CLEMENTS J, 1997, KININ SYSTEM, P71
[6]   VARIATIONS IN UTERINE KALLIKREIN DURING CYCLE AND EARLY-PREGNANCY IN THE RAT [J].
CORTHORN, J ;
VALDES, G .
BIOLOGY OF REPRODUCTION, 1994, 50 (06) :1261-1264
[7]   Estrogen and luminal stimulation of rat uterine kallikrein [J].
Corthorn, J ;
Figueroa, C ;
Valdes, G .
BIOLOGY OF REPRODUCTION, 1997, 56 (06) :1432-1438
[8]   EMBRYONIC SIGNALS IN PREGNANCY [J].
DEY, SK ;
JOHNSON, DC .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1986, 476 :49-62
[9]   Temporospatial changes of kinin B2 receptors during the estrous cycle and pregnancy in the rat uterus [J].
Figueroa, CD ;
Chacón, C ;
Corthorn, J ;
Ehrenfeld, P ;
Müller-Esterl, W ;
Valdés, G .
BIOLOGY OF REPRODUCTION, 2001, 64 (06) :1590-1599
[10]   BLOOD-FLOW TO UTERI OF SOWS DURING THE ESTROUS-CYCLE AND EARLY-PREGNANCY - LOCAL EFFECT OF THE CONCEPTUS ON THE UTERINE BLOOD-SUPPLY [J].
FORD, SP ;
CHRISTENSON, RK .
BIOLOGY OF REPRODUCTION, 1979, 21 (03) :617-624