Effect of lysophosphatidic acid on the ovum transport in mouse oviducts

被引：36

作者：

Kunikata, K

Yamano, S

Tokumura, A

Aono, T

机构：

[1] Univ Tokushima, Sch Med, Dept Obstet & Gynecol, Tokushima 7708503, Japan

[2] Univ Tokushima, Fac Pharmaceut Sci, Tokushima 7708505, Japan

来源：

LIFE SCIENCES | 1999年 / 65卷 / 08期

关键词：

ovum transport; lysophosphatidic acid; pertussis toxin; verapamil; mouse;

D O I：

10.1016/S0024-3205(99)00310-0

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The effects of lysophosphatidic acid (LPA) on ovum transport in mouse oviducts were studied. When excised oviducts were incubated at 37 degrees C under 5% CO2 in humidified air for 24 hours, addition of LPA at 10 mu M to the medium significantly accelerated the rate of ovum transport, and 1 mu M LPA slightly increased the ovum transport rate, These increases were not inhibited by 10 mu M indomethacin, a cyclooxygense inhibitor, but were suppressed by 200 ng/ml of pertussis toxin or 10 mu M verapamil, a voltage-sensitive calcium channel blocker. These data suggested that LPA stimulates mouse ovum transport by contracting oviductual smooth muscle via a voltage-sensitive calcium channel mediated by a pertussis toxin-sensitive G-protein-linked receptor.

引用

页码：833 / 840

页数：8

共 29 条

[1] Characterization of a novel subtype of human G protein-coupled receptor for lysophosphatidic acid [J].