Genotyping and quantitation of noroviruses in oysters from Two Distinct Sea areas in Japan

被引:60
作者
Nishida, Tomoko
Nishio, Osamu
Kato, Masahiko
Chuma, Takehisa
Kato, Hirotomo
Iwata, Hiroyuki
Kimura, Hirokazu
机构
[1] Yamaguchi Prefectural Res Inst Publ Hlth, Yamaguchi 7530821, Japan
[2] Natl Inst Infect Dis, Infect Dis Surveillance Ctr, Tokyo 2010011, Japan
[3] Gunma Prefectural Inst Publ Hlth & Environm Sci, Maebashi, Gunma 3710052, Japan
[4] Kagoshima Univ, Dept Vet Hyg, Kagoshima 8900065, Japan
[5] Yamaguchi Univ, Dept Vet Hyg, Yamaguchi 7538511, Japan
关键词
oyster; norovirus; phylogenetic analysis; real-time PCR; NORWALK-LIKE VIRUSES; MOLECULAR EPIDEMIOLOGY; PHYLOGENETIC ANALYSIS; GENETIC-ANALYSIS; ENTERIC VIRUSES; GASTROENTERITIS; SHELLFISH; OUTBREAKS; CONTAMINATION; SEWAGE;
D O I
10.1111/j.1348-0421.2007.tb03899.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Norovirus (NV) is a causative agent of acute gastroenteritis in humans, and shellfishes including oysters act as major vehicles of the virus. To investigate the genetic characteristics of NVs, we collected 1,512 oysters for raw consumption between October 2002 and March 2005 from two distinct areas (area A: the Sanriku Sea area; area B: the Setouchi Sea area). We detected the capsid gene and subjected it to phylogenetic analysis. By further quantification of the copy number of the genome by using real-time PCR, the NV capcid gene was detected in approximately 5% of the oysters, and they showed wide diversity. Two percent of the oysters from area B showed relatively large number of NVs, i.e., over 100 copies of capsid gene/oyster, whereas this was not observed in area A. Most of the detected NVs from oysters and humans were genetically related when the capsid region was compared. These results suggested that NVs obtained from humans and those obtained from oysters showed a potential relationship to each other and that some populations of Japanese oysters accumulated a relatively large number of NVs.
引用
收藏
页码:177 / 184
页数:8
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