Regulation of ILT3 gene expression by processing of precursor transcripts in human endothelial cells

被引：36

作者：

Kim-Schulze, S

Seki, T

Vlad, G

Scotto, L

Fan, J

Colombo, RC

Liu, J

Cortesini, R

Suciu-Foca, N ^{[1
]}

机构：

[1] Columbia Univ, Dept Pathol, New York, NY 10027 USA

[2] Columbia Univ, Dept Surg, New York, NY 10027 USA

[3] Columbia Univ, Dept Med, New York, NY 10027 USA

来源：

AMERICAN JOURNAL OF TRANSPLANTATION | 2006年 / 6卷 / 01期

关键词：

activation-induced splicing; immunoglobulin-like transcript 3; tolerogenic endothelial cells;

D O I：

10.1111/j.1600-6143.2005.01162.x

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Immunoglobulin-like transcript (ILT)-3 is a transmembrane receptor, which belongs to the immunoglobulin superfamily. In previous studies, we showed that allospecific CD8(+)CD28(-) T suppressor cells (Ts) induce the expression of ILT3 in human endothelial cells (EC) rendering them tolerogenic. Using a polymerase chain reaction (PCR)-based approach, we now demonstrate by cell fractionation and sequencing studies that ILT3 precursor RNA is expressed and retained in nuclei of resting EC. Ts interaction with EC or exposure of EC to interleukin-10 (IL-10) and interferon alpha (IFN-alpha) triggers processing of ILT3 pre-mRNA. Western blot analysis showed that the expression of the mature ILT3 transcript is accompanied by production of ILT3 protein.

引用

页码：76 / 82

页数：7

共 37 条

[1]

Alberti L, 2005, CANCER RES, V65, P2

[2] Anti-HLA antibody ligation to HLA class I molecules expressed by endothelial cells stimulates tyrosine phosphorylation, inositol phosphate generation, and proliferation [J].