Molecular genetic characterisation of whiB3, a mycobacterial homologue of a Streptomyces sporulation factor

被引：21

作者：

Hutter, B ^{[1
]}

Dick, T ^{[1
]}

机构：

[1] Inst Mol & Cell Biol, Singapore 117609, Singapore

来源：

RESEARCH IN MICROBIOLOGY | 1999年 / 150卷 / 05期

关键词：

sporulation; gene whiB; Streptomyces coelicolor; Mycobacterium smegmatis; bacterial growth; gene disruption;

D O I：

10.1016/S0923-2508(99)80055-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

WhiB is an essential sporulation factor in Streptomyces coelicolor. We report here the molecular genetic characterisation of whiB3, a whiB-like gene in the nonspore-forming Mycobacterium smegmatis mc(2)155. M. smegmatis whiB3 encodes a 96-amino-acid protein with 81% similarity to its M. tuberculosis counterpart identified in the genome project, and 35% similarity to S. coelicolor WhiB. In both mycobacteria, whiB3 is flanked by the same upstream gene, Rv3415c, and appears to be monocistronic. Promoter probe analyses suggest that the whiB3 gene is expressed constitutively. Disruption of whiB3 did not affect growth or the dormancy response of M. smegmatis. (C) Elsevier, Paris.

引用

页码：295 / 301

页数：7

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