Pre- and postnatal lung development, maturation, and plasticity - Cyclic mechanical stretch inhibits cell proliferation and induces apoptosis in fetal rat lung fibroblasts

被引:65
作者
Sanchez-Esteban, J
Wang, YL
Cicchiello, LA
Rubin, LP
机构
[1] Women & Infants Hosp Rhode Isl, Dept Pediat, Providence, RI 02905 USA
[2] Brown Univ, Sch Med, Providence, RI 02905 USA
关键词
flow cytometry; 5-bromo-2 '-deoxyuridine incorporation; programmed cell death; terminal deoxynucleotidyl transferase-mediated; dUTP-FITC nick-end labeling assay; caspase-3;
D O I
10.1152/ajplung.00399.2000
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Development of the pulmonary air sacs is crucial for extrauterine survival. Late fetal lung development is characterized by a thinning of the mesenchyme, which brings pneumocytes and endothelial cells into apposition. We hypothesized that mechanical stretch, simulating fetal breathing movements, plays an important role in this remodeling process. Using a Flexercell Strain Unit, we analyzed the effects of intermittent stretch on cell proliferation and apoptosis activation in fibroblasts isolated from fetal rat lungs during late development. On day 19, intermittent stretch increased cells in G(0)/G(1) by 22% (P = 0.001) and decreased in S phase by 50% (P = 0.003) compared with unstretched controls. Cell proliferation analyzed by 5-bromo-2'-deoxyuridine incorporation showed a similar magnitude of cell cycle arrest (P = 0.04). At this same gestational age, stretch induced apoptosis by two- to threefold over controls, assayed by DNA flow cytometry, terminal deoxynucleotidyl transferase-mediated dUTP-FITC nick-end labeling, and caspase-3 activation. These results indicate that mechanical stretch of fibroblasts isolated during the canalicular stage inhibits cell cycle progression and activates apoptosis. These findings are cotemporal with the mesenchymal thinning that normally occurs in situ.
引用
收藏
页码:L448 / L456
页数:9
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