Biodiesel production from sunflower, soybean, and waste cooking oils by transesterification using lipase immobilized onto a novel microporous polymer

被引:194
作者
Dizge, Nadir [1 ]
Aydiner, Coskun [1 ]
Imer, Derya Y. [1 ]
Bayramoglu, Mahmut [2 ]
Tanriseven, Aziz [3 ]
Keskinlera, Buelent [1 ]
机构
[1] Gebze Inst Technol, Dept Environm Engn, TR-41400 Gebze, Turkey
[2] Gebze Inst Technol, Dept Chem Engn, TR-41400 Gebze, Turkey
[3] Gebze Inst Technol, Dept Biochem, TR-41400 Gebze, Turkey
关键词
Biodiesel; Transesterification; Microporous polymeric biocatalyst; Thermomyces lanuginosus lipase; Taguchi methodology; ACETALDEHYDE; OPTIMIZATION;
D O I
10.1016/j.biortech.2008.10.008
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
This study aims at carrying out lipase-catalyzed synthesis of fatty acid methyl esters (biodiesel) from various vegetable oils using lipase immobilized onto a novel microporous polymeric matrix (MPPM) as a low-cost biocatalyst. The research is focused on three aspects of the process: (a) MPPM synthesis (monolithic, bead, and powder forms), (b) microporous polymeric biocatalyst (MPPB) preparation by immobilization of lipase onto MPPM, and (c) biodiesel production by MPPB. Experimental planning of each step of the study was separately carried out in accordance with design of experiment (DoE) based on Taguchi methodology. Microporous polymeric matrix (MPPM) containing aldehyde functional group was synthesized by polyHIPE technique using styrene, divinylbenzene, and polyglutaraldehyde. Thermomyces lanuginosus lipase was covalently attached onto MPPM with 80%, 85%, and 89% immobilization efficiencies using bead, powder, and monolithic forms, respectively. Immobilized enzymes were successfully used for the production of biodiesel using sunflower, soybean, and waste cooking oils. It was shown that immobilized enzymes retain their activities during 10 repeated batch reactions at 25 degrees C, each lasting 24 h. Since the developed novel method is simple yet effective, it could have a potential to be used industrially for the production of chemicals requiring immobilized lipases. (c) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1983 / 1991
页数:9
相关论文
共 27 条
[1]  
[Anonymous], 2004, INT J PRODUCT PERFOR, DOI DOI 10.1108/17410400410569125
[2]  
[Anonymous], 2003, 14103 DIN EN
[3]  
[Anonymous], 2062 ISO
[4]  
*AOCS, 1998, 125 CD AOCS
[5]   Immobilization of glucoamylase onto spacer-arm attached magnetic poly(methylmethacrylate) microspheres: characterization and application to a continuous flow reactor [J].
Arica, MY ;
Yavuz, H ;
Patir, S ;
Denizli, A .
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2000, 11 (2-3) :127-138
[6]  
BHUMGARA ZG, 1995, THESIS U EXETER
[7]   Determination of the sites of 4-hydroxy-2-nonenal adduction to protein by electrospray tandem mass spectrometry [J].
Bolgar, MS ;
Gaskell, SJ .
ANALYTICAL CHEMISTRY, 1996, 68 (14) :2325-2330
[8]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[9]   Application of ANOVA method to precipitation behaviour studies [J].
Cvijovic, Z ;
Radenkovic, B ;
Maksimovic, V ;
Dimcic, B .
MATERIALS SCIENCE AND ENGINEERING A-STRUCTURAL MATERIALS PROPERTIES MICROSTRUCTURE AND PROCESSING, 2005, 397 (1-2) :195-203
[10]   Synthesis and functionalisation of polyHIPE® beads [J].
Desforges, A ;
Arpontet, M ;
Deleuze, H ;
Mondain-Monval, O .
REACTIVE & FUNCTIONAL POLYMERS, 2002, 53 (2-3) :183-192