Cell-free DNA in the plasma of patients with systemic sclerosis

被引：26

作者：

Mosca, Marta ^{[1
]}

Giuliano, Tiziana ^{[1
]}

Cuomo, Giovanna ^{[2
]}

Doveri, Marica ^{[1
]}

Tani, Chiara ^{[1
]}

Curcio, Michele ^{[3
]}

Abignano, Giuseppina ^{[2
]}

De Feo, Francesca ^{[1
]}

Bazzichi, Laura ^{[1
]}

Della Rossa, Alessandra ^{[1
]}

Valentini, Gabriele ^{[2
]}

Bombardieri, Stefano ^{[1
]}

机构：

[1] Univ Pisa, Rheumatol Unit, Dept Internal Med, I-56126 Pisa, Italy

[2] Univ Naples 2, Rheumatol Unit, Naples, Italy

[3] Univ Pisana, UO Immunoematol, Azienda Osped 2, Pisa, Italy

来源：

CLINICAL RHEUMATOLOGY | 2009年 / 28卷 / 12期

关键词：

Biomarkers; Cell-free DNA; Disease activity; Plasma cell-free DNA; Scleroderma; Systemic autoimmune diseases; CIRCULATING DNA; DISEASE-ACTIVITY; FETAL DNA; SERUM; ORIGIN; WOMEN;

D O I：

10.1007/s10067-009-1245-5

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The aim of the present study was to evaluate the concentration of cell-free DNA (cf-DNA) in the plasma of patients with systemic sclerosis (SSc) and to examine the correlation of cf-DNA with clinical variables of the disease. The study population consisted of 122 SSc patients and 16 healthy controls. Epidemiological and clinical data were collected by direct assessment. The beta-globin gene was used to determine the total amount of DNA in the plasma by real-time quantitative PCR analysis. cf-DNA was found in all patients (mean concentration 1,420.7 copies/ml) and controls (mean concentration 1,462.5), with no significant difference. In SSc patients, no correlation was found between cf-DNA and the type of organ involvement, but patients with active disease presented significantly higher cf-DNA concentrations than those with inactive disease (p < 0.05). Our data suggest that cf-DNA could provide a useful biomarker for the assessment of disease activity in SSc patients.

引用

页码：1437 / 1440

页数：4

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