p53 suppresses the Nrf2-dependent transcription of antioxidant response genes

被引:291
作者
Faraonio, Raffaella
Vergara, Paola
Di Marzo, Domenico
Pierantoni, Maria Giovanna
Napolitano, Maria
Russo, Tommaso [1 ]
Cimino, Filiberto
机构
[1] Univ Naples Federico II, Dipartimento Biochim & Biotecnol Med, CEINGE Biotecnol Avanzate, I-80131 Naples, Italy
[2] Univ Naples Federico II, Dipartimento Biol & Patol Cellulare & Mol, I-80131 Naples, Italy
[3] Ist Nazl Tumori, Fdn Pascale, I-80131 Naples, Italy
关键词
D O I
10.1074/jbc.M605707200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cells respond to the shift of intracellular environment toward pro-oxidant conditions by activating the transcription of numerous "antioxidant" genes. This response is based on the activation of the Nrf2 transcription factor, which transactivates the genes containing in their promoters the antioxidant response cis- elements (AREs). If the oxidative stress provokes DNA damage, a second response of the cell takes place, based on the activation of p53, which induces cell cycle arrest and/or apoptosis. Here we have explored the cross-talk between these two regulatory mechanisms. The results show that p53 counteracts the Nrf2-induced transcription of three ARE-containing promoters of the x-CT, NQO1, and GST-alpha 1 genes. Endogenous transcripts of these antioxidant genes accumulate as a consequence of Nrf2 overexpression or exposure to electrophile diethylmaleate, but these effects are again blocked by p53 overexpression or endogenous p53 activation. Chromatin immuno-precipitation experiments support the hypothesis that this p53-dependent trans-repression is due to the direct interaction of p53 with the ARE-containing promoters. Considering that p53-induced apoptosis requires an accumulation of reactive oxygen species, this negative control on the Nrf2 transactivation appears to be aimed to prevent the generation of a strong antioxidant intracellular environment that could hinder the induction of apoptosis.
引用
收藏
页码:39776 / 39784
页数:9
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