Measurement of alcohol acetyltransferase and ester hydrolase activities in yeast extracts

被引：27

作者：

Rojas, V

Gil, JV ^{[1
]}

Manzanares, P

Gavara, R

Piñaga, F

Flors, A

机构：

[1] Dept Biotecnol Alimentos, Valencia 46100, Spain

[2] CSIC, Inst Agroquim & Tecnol Alimentos, Dept Conservac & Calidad Alimentos, Valencia 46100, Spain

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2002年 / 30卷 / 02期

关键词：

alcohol acetyltransferase; ester hydrolase; headspace-SPME;

D O I：

10.1016/S0141-0229(01)00483-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A method has been developed for measurement in vitro of the simultaneous activities alcohol acetyltransferase (AATase) and ester hydrolase (EHase) in yeast extracts taking into account the possibility of interaction between the two opposite activities or the rapid inactivation of the AATase activity. A mathematical model. including as parameters a first order kinetic constant corresponding to the EHase activity and the inactivation constant of AATase. is proposed for the evaluation of AATase activity. To determine ester concentrations, the Headspace-SPME-GC technique has been used. The method has been successfully applied to three yeast strains belonging to the species Pichia anomala, Pichia heedii and Saccharomyces cerevisiae and the corresponding parameters for AATase and EHase have been calculated. (C) 2002 Elsevier Science Inc. All rights reserved.

引用

页码：224 / 230

页数：7

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