Loop-Mediated Isothermal Amplification Assay Targeting the femA Gene for Rapid Detection of Staphylococcus aureus from Clinical and Food Samples

被引：32

作者：

Zhao Xihong ^{[1
,2
,3
,4
]}

Li, Yanmei ^{[5
,6
]}

Park, Myoungsu ^{[3
,4
]}

Wang, Jun ^{[3
,4
]}

Zhang, Youhong ^{[1
]}

He, Xiaowei ^{[2
]}

Forghani, Fereidoun ^{[3
,4
]}

Wang, Li ^{[7
]}

Yu, Guangchao ^{[8
]}

Oh, Deog-Hwan ^{[3
,4
]}

机构：

[1] Wuhan Inst Technol, Sch Chem Engn & Pharm, Minist Educ, Key Lab Green Chem Proc, Wuhan 430073, Peoples R China

[2] S China Univ Technol, Coll Light Ind & Food Sci, Guangzhou 510640, Peoples R China

[3] Kangwon Natl Univ, Dept Food Sci & Biotechnol, Chunchon 200701, South Korea

[4] Kangwon Natl Univ, Inst Biosci & Biotechnol, Chunchon 200701, South Korea

[5] Guangzhou Women & Childrens Med Ctr, Guangzhou 510623, Guangdong, Peoples R China

[6] Univ Maryland, Sch Dent, Dept Microbial Pathogenesis, Baltimore, MD 21201 USA

[7] South China Agr Univ, Coll Food Sci, Food Safety Key Lab Guangdong Prov, Guangzhou 510642, Guangdong, Peoples R China

[8] Jinan Univ, Affiliated Hosp 1, Guangzhou 510620, Guangdong, Peoples R China

来源：

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2013年 / 23卷 / 02期

基金：

美国国家科学基金会;

关键词：

Loop-mediated isothermal amplification (LAMP); Staphylococcus aureus; femA; rapid detection; clinical samples; food samples; INTEGRON; STRAINS; CHINA; PCR;

D O I：

10.4014/jmb.1207.07022

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, a loop-mediated isothermal amplification (LAMP) method to rapidly detect Staphylococcus aureus strains was developed and evaluated by extensively applying a large number of S. aureus isolates from clinical and food samples. Six primers were specially designed for recognizing eight distinct sequences on the species-specific femA gene of S. aureus. The detection limits were 100 fg DNA/tube and 10(4) CFU/ml. The LAMP assay was applied to 432 S. aureus strains isolated from 118 clinical and 314 food samples. Total detection rates for the LAMP and polymerase chain reaction assays were 98.4% (306/311) and 89.4% (278/311), respectively.

引用

页码：246 / 250

页数：5

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