A molecular atlas of cell types and zonation in the brain vasculature

被引：1255

作者：

Vanlandewijck, Michael ^{[1
,3
]}

He, Liqun ^{[2
]}

Mae, Maarj A. Andaloussi ^{[3
]}

Andrae, Johanna ^{[3
]}

Ando, Koji ^{[3
]}

Del Gaudio, Francesca ^{[4
]}

Nahar, Khayrun ^{[3
]}

Lebouvier, Thibaud ^{[3
,5
]}

Lavina, Barbara ^{[3
]}

Gouveia, Leonor ^{[3
]}

Sun, Ying ^{[6
]}

Raschperger, Elisabeth ^{[1
]}

Rasanen, Markus ^{[7
,8
]}

Zarb, Yvette ^{[9
]}

Mochizuki, Naoki ^{[10
,11
]}

Keller, Annika ^{[9
]}

Lendahl, Urban ^{[4
]}

Betsholtz, Christer ^{[1
,3
]}

机构：

[1] Karolinska Inst, AstraZeneca Integrated Cardio Metab Ctr KI AZ ICM, Blickagangen 6, SE-14157 Huddinge, Sweden

[2] Tianjin Med Univ, Key Lab Postneuroinjury Neurorepair & Regenerat C, Dept Neurosurg,Gen Hosp, Tianjin Neurol Inst,Minist Educ & Tianjin City, Tianjin 300052, Peoples R China

[3] Uppsala Univ, Rudbeck Lab, Dept Immunol Genet & Pathol, Dag Hammarskjolds Vag 20, SE-75185 Uppsala, Sweden

[4] Karolinska Inst, Dept Cell & Mol Biol, Von Eulers Vag 3, SE-17177 Stockholm, Sweden

[5] Univ Lille, CHU,Memory Ctr, Distalz, Inserm,U1171, F-59000 Lille, France

[6] Zhongyuan Union Genet Technol Co Ltd, Dept Bioinformat, Tianjin Airport Econ Area, 45 9th East Rd, Tianjin 300304, Peoples R China

[7] Univ Helsinki, Wihuri Res Inst, Haartmaninkatu 8,POB 63, FI-00014 Helsinki, Finland

[8] Univ Helsinki, Translat Canc Biol Program, Biomedicum Helsinki, Haartmaninkatu 8,POB 63, FI-00014 Helsinki, Finland

[9] Zurich Univ, Univ Zurich Hosp, Div Neurosurg, CH-8091 Zurich, Switzerland

[10] Natl Cerebral & Cardiovasc Ctr, Dept Cell Biol, Res Inst, Suita, Osaka, Japan

[11] Natl Cerebral & Cardiovasc Ctr, AMED CREST, Suita, Osaka, Japan

来源：

NATURE | 2018年 / 554卷 / 7693期

基金：

瑞典研究理事会; 瑞士国家科学基金会; 欧洲研究理事会;

关键词：

MESENCHYMAL STEM-CELLS; BMX TYROSINE KINASE; ENDOTHELIAL-CELLS; GENE-EXPRESSION; BARRIER TRANSPORT; BLOOD-FLOW; RNA-SEQ; PERICYTES; ORIGIN; EFFLUX;

D O I：

10.1038/nature25739

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cerebrovascular disease is the third most common cause of death in developed countries, but our understanding of the cells that compose the cerebral vasculature is limited. Here, using vascular single-cell transcriptomics, we provide molecular definitions for the principal types of blood vascular and vessel-associated cells in the adult mouse brain. We uncover the transcriptional basis of the gradual phenotypic change (zonation) along the arteriovenous axis and reveal unexpected cell type differences: a seamless continuum for endothelial cells versus a punctuated continuum for mural cells. We also provide insight into pericyte organotypicity and define a population of perivascular fibroblast-like cells that are present on all vessel types except capillaries. Our work illustrates the power of single-cell transcriptomics to decode the higher organizational principles of a tissue and may provide the initial chapter in a molecular encyclopaedia of the mammalian vasculature.

引用

页码：475 / +

页数：34

共 63 条

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