A gene encoding multidrug resistance (MDR)-like protein is induced by aluminum and inhibitors of calcium flux in wheat

被引:59
作者
Sasaki, T
Ezaki, B
Matsumoto, H
机构
[1] Okayama Univ, Bioresources Res Inst, Kurashiki, Okayama 7100046, Japan
[2] BRAIN, Biooriented Technol Res Adv Inst, Saitama 3318537, Japan
关键词
ABC protein; aluminum stress; calcium homeostasis; MDR wheat (Triticum aestivum L.);
D O I
10.1093/pcp/pcf025
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A cDNA clone exclusively induced by aluminum (Al) was isolated from root apices of wheat (Triticum aestivum L.) by the differential display method. The predicted amino acid sequence exhibited homology to the multidrug resistance (MDR) proteins that is known as a member of the ATP-binding cassette (ABC) protein superfamily. Thus this gene was named TaMDR1 (Triticum aestivum MDR). TaMDR1 was induced as a function of Al concentration in the range from 5 to 50 muM, which is in the range of Al content in natural acid soil environment. The concentration required for the induction was lower in the Al-sensitive cultivar than in the Al-tolerant cultivar, indicating that the accumulation of TaMDR1 mRNA was associated with the events caused by Al toxicity rather than Al tolerance. TaMDR1 was significantly induced by the exposure to lanthanum, gadolinium and ruthenium red, which are known as inhibitors of calcium channels. Furthermore, decreasing of calcium ion in growth medium caused stimulation of the gene expression. These results suggested that the induction of TaMDR1 is caused by the breaking of calcium homeostasis which occurred at early stage of Al toxicity.
引用
收藏
页码:177 / 185
页数:9
相关论文
共 44 条
[1]   THE GENOMIC INHERITANCE OF ALUMINUM TOLERANCE IN ATLAS-66 WHEAT [J].
BERZONSKY, WA .
GENOME, 1992, 35 (04) :689-693
[2]   ABC transporters in lipid transport [J].
Borst, P ;
Zelcer, N ;
van Helvoort, A .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 2000, 1486 (01) :128-144
[3]   INTERNAL DUPLICATION AND HOMOLOGY WITH BACTERIAL TRANSPORT PROTEINS IN THE MDR1 (P-GLYCOPROTEIN) GENE FROM MULTIDRUG-RESISTANT HUMAN-CELLS [J].
CHEN, CJ ;
CHIN, JE ;
UEDA, K ;
CLARK, DP ;
PASTAN, I ;
GOTTESMAN, MM ;
RONINSON, IB .
CELL, 1986, 47 (03) :381-389
[4]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[5]   cDNA clones encoding 1,3-beta-glucanase and a fimbrin-like cytoskeletal protein are induced by Al toxicity in wheat roots [J].
CruzOrtega, R ;
Cushman, JC ;
Ownby, JD .
PLANT PHYSIOLOGY, 1997, 114 (04) :1453-1460
[6]   Cloning and characterisation of a novel P-glycoprotein homologue from barley [J].
Davies, TGE ;
Theodoulou, FL ;
Hallahan, DL ;
Forde, BG .
GENE, 1997, 199 (1-2) :195-202
[7]   Aluminum tolerance in transgenic plants by alteration of citrate synthesis [J].
delaFuente, JM ;
RamirezRodriguez, V ;
CabreraPonce, JL ;
HerreraEstrella, L .
SCIENCE, 1997, 276 (5318) :1566-1568
[8]   ALUMINUM TOLERANCE IN WHEAT (TRITICUM-AESTIVUM L) .2. ALUMINUM-STIMULATED EXCRETION OF MALIC-ACID FROM ROOT APICES [J].
DELHAIZE, E ;
RYAN, PR ;
RANDALL, PJ .
PLANT PHYSIOLOGY, 1993, 103 (03) :695-702
[9]   ALUMINUM TOXICITY AND TOLERANCE IN PLANTS [J].
DELHAIZE, E ;
RYAN, PR .
PLANT PHYSIOLOGY, 1995, 107 (02) :315-321
[10]   ALUMINUM TOLERANCE IN WHEAT (TRITICUM-AESTIVUM L) .1. UPTAKE AND DISTRIBUTION OF ALUMINUM IN ROOT APICES [J].
DELHAIZE, E ;
CRAIG, S ;
BEATON, CD ;
BENNET, RJ ;
JAGADISH, VC ;
RANDALL, PJ .
PLANT PHYSIOLOGY, 1993, 103 (03) :685-693