Sensitive red protein calcium indicators for imaging neural activity

被引：720

作者：

Dana, Hod ^{[1
]}

Mohar, Boaz ^{[1
,2
]}

Sun, Yi ^{[1
]}

Narayan, Sujatha ^{[1
]}

Gordus, Andrew ^{[3
]}

Hasseman, Jeremy P. ^{[1
]}

Tsegaye, Getahun ^{[1
]}

Holt, Graham T. ^{[1
]}

Hu, Amy ^{[1
]}

Walpita, Deepika ^{[1
]}

Patel, Ronak ^{[1
]}

Macklin, John J. ^{[1
]}

Bargmann, Cornelia I. ^{[3
]}

Ahrens, Misha B. ^{[1
]}

Schreiter, Eric R. ^{[1
]}

Jayaraman, Vivek ^{[1
]}

Looger, Loren L. ^{[1
]}

Svoboda, Karel ^{[1
]}

Kim, Douglas S. ^{[1
]}

机构：

[1] Howard Hughes Med Inst, Janelia Res Campus, Ashburn, VA 20147 USA

[2] Weizmann Inst Sci, Rehovot, Israel

[3] Rockefeller Univ, Howard Hughes Med Inst, New York, NY 10021 USA

来源：

ELIFE | 2016年 / 5卷

关键词：

IN-VIVO; CRE-RECOMBINASE; MOTOR CORTEX; 2-PHOTON; ORANGE; OPTIMIZATION; RESPONSES; SOFTWARE; CIRCUIT;

D O I：

10.7554/eLife.12727

中图分类号：

Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程];

摘要：

Genetically encoded calcium indicators (GEC's) allow measurement of activity in large populations of neurons and in small neuronal compartments, over times of milliseconds to months. Although GFP-based GEC's are widely used for in vivo neurophysiology, GEC's with red-shifted excitation and emission spectra have advantages for in vivo imaging because of reduced scattering and absorption in tissue, and a consequent reduction in phototoxicity. However, current red GEC's are inferior to the state-of-the-art GFP-based GCaMP6 indicators for detecting and quantifying neural activity. Here we present improved red GEC's based on mRuby (jRCaMP1a, b) and mApple (jRGECO1a), with sensitivity comparable to GCaMP6. We characterized the performance of the new red GEC's in cultured neurons and in mouse, Drosophila, zebrafish and C. elegans in vivo. Red GEC's facilitate deep-tissue imaging, dual-color imaging together with GFP-based reporters, and the use of optogenetics in combination with calcium imaging.

引用

页数：24

共 51 条

[1]

Ahrens MB, 2013, NAT METHODS, V10, P413, DOI [10.1038/NMETH.2434, 10.1038/nmeth.2434]

[2]

Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics [J].

Akerboom, Jasper ;

Calderon, Nicole Carreras ;

Tian, Lin ;

Wabnig, Sebastian ;

Prigge, Matthias ;

Tolo, Johan ;

Gordus, Andrew ;

Orger, Michael B. ;

Severi, Kristen E. ;

Macklin, John J. ;

Patel, Ronak ;

Pulver, Stefan R. ;

Wardill, Trevor J. ;

Fischer, Elisabeth ;

Schueler, Christina ;

Chen, Tsai-Wen ;

Sarkisyan, Karen S. ;

Marvin, Jonathan S. ;

Bargmann, Cornelia I. ;

Kim, Douglas S. ;

Kugler, Sebastian ;

Lagnado, Leon ;

Hegemann, Peter ;

Gottschalk, Alexander ;

Schreiter, Eric R. ;

Looger, Loren L. .

FRONTIERS IN MOLECULAR NEUROSCIENCE, 2013, 6

[3]

Optimization of a GCaMP Calcium Indicator for Neural Activity Imaging [J].

Akerboom, Jasper ;

Chen, Tsai-Wen ;

Wardill, Trevor J. ;

Tian, Lin ;

Marvin, Jonathan S. ;

Mutlu, Sevinc ;

Calderon, Nicole Carreras ;

Esposti, Federico ;

Borghuis, Bart G. ;

Sun, Xiaonan Richard ;

Gordus, Andrew ;

Orger, Michael B. ;

Portugues, Ruben ;

Engert, Florian ;

Macklin, John J. ;

Filosa, Alessandro ;

Aggarwal, Aman ;

Kerr, Rex A. ;

Takagi, Ryousuke ;

Kracun, Sebastian ;

Shigetomi, Eiji ;

Khakh, Baljit S. ;

Baier, Herwig ;

Lagnado, Leon ;

Wang, Samuel S. -H. ;

Bargmann, Cornelia I. ;

Kimmel, Bruce E. ;

Jayaraman, Vivek ;

Svoboda, Karel ;

Kim, Douglas S. ;

Schreiter, Eric R. ;

Looger, Loren L. .

JOURNAL OF NEUROSCIENCE, 2012, 32 (40) :13819-13840

[4]

Crystal Structures of the GCaMP Calcium Sensor Reveal the Mechanism of Fluorescence Signal Change and Aid Rational Design [J].

Akerboom, Jasper ;

Rivera, Jonathan D. Velez ;

Guilbe, Maria M. Rodriguez ;

Malave, Elisa C. Alfaro ;

Hernandez, Hector H. ;

Tian, Lin ;

Hires, S. Andrew ;

Marvin, Jonathan S. ;

Looger, Loren L. ;

Schreiter, Eric R. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (10) :6455-6464

[5]

Functional Specialization of Mouse Higher Visual Cortical Areas [J].

Andermann, Mark L. ;

Kerlin, Aaron M. ;

Roumis, Demetris K. ;

Glickfeld, Lindsey L. ;

Reid, R. Clay .

NEURON, 2011, 72 (06) :1025-1039

[6]

The psychophysics toolbox [J].

Brainard, DH .

SPATIAL VISION, 1997, 10 (04) :433-436

[7]

BRENNER S, 1974, GENETICS, V77, P71

[8]

Cai D, 2013, NAT METHODS, V10, P540, DOI [10.1038/NMETH.2450, 10.1038/nmeth.2450]

[9]

Behaviour-dependent recruitment of long-range projection neurons in somatosensory cortex [J].

Chen, Jerry L. ;

Carta, Stefano ;

Soldado-Magraner, Joana ;

Schneider, Bernard L. ;

Helmchen, Fritjof .

NATURE, 2013, 499 (7458) :336-+

[10]

Ultrasensitive fluorescent proteins for imaging neuronal activity [J].

Chen, Tsai-Wen ;

Wardill, Trevor J. ;

Sun, Yi ;

Pulver, Stefan R. ;

Renninger, Sabine L. ;

Baohan, Amy ;

Schreiter, Eric R. ;

Kerr, Rex A. ;

Orger, Michael B. ;

Jayaraman, Vivek ;

Looger, Loren L. ;

Svoboda, Karel ;

Kim, Douglas S. .

NATURE, 2013, 499 (7458) :295-+

← 1 2 3 4 5 6 →