Characterization of digestive enzyme activity during larval development of gilthead seabream (Sparus aurata)

被引：248

作者：

Moyano, FJ ^{[1
]}

Diaz, M ^{[1
]}

Alarcon, FJ ^{[1
]}

Sarasquete, MC ^{[1
]}

机构：

[1] INST CIENCIAS MARINAS ANDALUCIA, POLIGONO RION SAN PEDRO, CADIZ, SPAIN

来源：

FISH PHYSIOLOGY AND BIOCHEMISTRY | 1996年 / 15卷 / 02期

关键词：

digestive enzymes; seabream; larvae; SDS-PAGE;

D O I：

10.1007/BF01875591

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The evolution of the digestive enzyme equipment in seabream from hatching to 30 days old larvae was studied; there was a progressive increase in the activity of protease, amylase and acid and alkaline phosphatase from day 15 onwards. The use of specific inhibitors, and SDS-PAGE provided evidence to suggest that most of the proteases belonged to the serine group. A high alpha-amylase activity was also denoted. Zymograms of larval extracts indicated that exogenous food has more a qualitative than a quantitative role in the secretion of digestive enzymes in this species.

引用

页码：121 / 130

页数：10

共 40 条

[1]

BARAGI V, 1986, T AM FISH SOC, V115, P478, DOI 10.1577/1548-8659(1986)115<478:DEAISB>2.0.CO

[2]

[3]

BONNIE SP, 1991, COMP BIOCH PHYSL, V100, P725

[4]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[5] EARLY WEANING OF SEA BASS (DICENTRARCHUS-LABRAX) LARVAE WITH A COMPOUND DIET - EFFECT ON DIGESTIVE ENZYMES [J].

CAHU, CL ;

INFANTE, JLZ .

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 1994, 109 (02) :213-222

[6] PROTEASE DEVELOPMENT IN DOVER SOLE [SOLEA-SOLEA (L)] [J].

CLARK, J ;

MURRAY, KR ;

STARK, JR .

AQUACULTURE, 1986, 53 (3-4) :253-262

[7]

CORNEILLE S, 1992, IOAN FISHF C GREEC

[8]

Corneille S., 1989, EAS SPECIAL PUBL, V10, P73

[9] ONTOGENY OF ENZYMATIC-ACTIVITIES IN FED AND FASTING TURBOT, SCOPHTHALMUS-MAXIMUS L [J].

COUSIN, JCB ;

BAUDINLAURENCIN, F ;

GABAUDAN, J .

JOURNAL OF FISH BIOLOGY, 1987, 30 (01) :15-33

[10] STUDY OF APPLICATION OF PROTEOLYTIC-ENZYMES TO FISH FOOD [J].

DABROWSKI, K ;

GLOGOWSKI, J .

AQUACULTURE, 1977, 12 (04) :349-360

← 1 2 3 4 →