Purification of benzylpenicillin filtered broths by ultrafiltration and effect on solvent extraction

被引：16

作者：

Nabais, AMA ^{[1
]}

Cardoso, JP

机构：

[1] Univ Tecn Lisboa, Engn Bioquim Lab, Inst Super Tecn, P-1096 Lisbon, Portugal

[2] CIPAN, SAVala Carregado, P-2600 Castanheira, Portugal

来源：

BIOPROCESS ENGINEERING | 1999年 / 21卷 / 02期

关键词：

Molecular Weight; Purification; Fermentation; Phase Separation; PVDF;

D O I：

10.1007/s004490050656

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Purification of benzylpenicillin filtered broths obtained from fermented broths produced at CIPAN, SA Pilot Plant Fermentation was carried out by ultrafiltration with diafiltration. This study was done using an ultrafiltration laboratory/pilot installation from Paterson Candy International Ltd. whose module has a tubular configuration. Membranes with molecular weight cut-offs of 8000 (polysulfone), 20 000 (polysulfone) and 100 000 (PVDF) were assessed. Comparison between solvent extraction of benzylpenicillin from ultrafiltered broths and from filtered broths using flocculants was also done. Ultrafiltration with diafiltration of benzylpenicillin filtered broths was used successfully to remove proteins, colored substances and other impurities giving high benzylpenicillin recovery in the permeate, as an alternative to the use of flocculants and anti-emulsion agents to obtain good phases separation in benzylpenicillin solvent extraction.

引用

页码：157 / 163

页数：7