Temperature dependence of internucleotide nitrogen-nitrogen scalar couplings in RNA

被引：9

作者：

Bytchenkoff, D

Chiarparin, E

Früh, D

Rüdisser, S

Bodenhausen, G

机构：

[1] Ecole Normale Super, Dept Chim, CNRS, F-75231 Paris 05, France

[2] Novartis Pharma AG, CH-4002 Basel, Switzerland

[3] Aventis Pharma, F-94403 Vitry Sur Seine, France

[4] Ecole Polytech Fed Lausanne, Inst Chim Mol & Biol, BCH, CH-1015 Lausanne, Switzerland

来源：

MAGNETIC RESONANCE IN CHEMISTRY | 2002年 / 40卷 / 05期

关键词：

RNA; Watson-Crick base pairs; scalar couplings across hydrogen bonds; base-pair mobility; temperature-dependent couplings;

D O I：

10.1002/mrc.1018

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The temperature dependence of internucleotide nitrogen-nitrogen scalar couplings (2h)J(N,N) across hydrogen bonds in adenine-uracil (A-U) and guanine - cytosine (G-C) base pairs of the 22 nucleotide RNA oligomer GGCGAAGUCGAAAGAUGGCGCC was studied between 280 and 310 K. The value of (2h)J(N,N) was observed to decrease monotonically for all four base pairs with increasing temperature. The temperature dependence of (2h)f(N,N) was found to be more pronounced for the A-U base pair than for G-C base pairs. An earlier study of cross-correlation effects at 296 K appeared to indicate a reduced mobility of the A-U base pair, as evidenced by small contributions of chemical shift modulation to relaxation rates. Copyright (C) 2002 John Wiley Sons, Ltd.

引用

页码：377 / 379

页数：3

共 17 条

[1] The chemical nature of hydrogen bonding in proteins via NMR:: J-couplings, chemical shifts, and AIM theory [J].