A pseudo-exponential feeding method for control of specific growth rate in fed-batch cultures

被引：26

作者：

Cheng, LC

Wu, JY

Chen, TL ^{[1
]}

机构：

[1] Natl Cheng Kung Univ, Dept Chem Engn, Tainan 701, Taiwan

[2] I Shou Univ, Dept Chem Engn, Kaohsiung 840, Taiwan

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2002年 / 10卷 / 03期

关键词：

fed-batch culture; feeding method; Escherichia coli;

D O I：

10.1016/S1369-703X(02)00002-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A simple feeding method for controlling specific growth rate in fed-batch culture was developed. This method applies a constant feed rate using a concentrate reservoir and two mixing chambers in series to simulate the exponential feeding. Fed-batch cultures with Escherichia coli showed that the present feeding method could sustain the cells growing at predetermined specific growth rates, where the time length for exponential growth was dependent on the magnitude of the growth rate. The present feeding method is convenient to operate, requires no computerized control equipments, and thus could expect an extensive application in fed-batch culture. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：227 / 232

页数：6

共 11 条

[1] Fed-batch culture of Escherichia coli W by exponential feeding of sucrose as a carbon source [J].